GC-MS/MS法测定聚乙二醇6000中4种常用抗氧剂的含量

Determination of 4 antioxidants in polyethylene glycol 6000 by GC-MS/MS

目的建立药用辅料聚乙二醇6000中4种抗氧剂的定量测定方法,为聚乙二醇6000的质量控制提供技术支撑。方法采用气质联用(GC-MS/MS)法同时检测聚乙二醇6000中2,6-二叔丁基对甲基苯酚(BHT)、叔丁基对羟基茴香醚(BHA)、叔丁基对苯二酚(TBHQ)、2,6-二叔丁基-4羟基苯酚(Ionox-100)4种抗氧剂的含量。使用DB-5 MS毛细管柱(30 m×0.25mm×0.25μm);多反应监测(MRM)模式。结果4种抗氧剂相关系数在0.9996~1.000;平均回收率(n=6)为92.7%~101.3%,RSD为2.2%~3.0%。结论该方法灵敏度高、专属性强,适用于聚乙二醇6000中4种抗氧剂的检测。

Objective To quantitatively determine 4 antioxidants in polyethylene glycol 6000 to provide a reference for the quality control of polyethylene glycol 6000.Methods The content of 4 antioxidants in polyethylene glycol 6000 was determined under the multiple reaction monitoring(MRM)mode by GC-MS/MS.The capillary column was DB-5 MS(30 m×0.25 mm×0.25μm).Results The 4 antioxidants of correlation coefficient values were at 0.9996~1.000.The average recovery ranged 92.7%~101.3%(n=6),and the RSD 2.2%~3.0%.Conclusion The method is sensitive and specific,which is suitable for the determination of 4 antioxidants in polyethylene glycol 6000.