咖啡豆乙醇部位的化学成分研究

Chemical constituents from ethanol fraction of coffee beans

综合应用HP-20大孔树脂柱层析、半制备HPLC等多种分离手段对咖啡豆乙醇部位进行分离纯化,共得到15个化合物,运用NMR、MS等现代波谱方法分别鉴定为咖啡因(1)、1,4-二-O-(E)-咖啡酰-β-D-吡喃葡萄糖苷(2)、1,3-O-二咖啡酰甘油(3)、咖啡酸(4)、咖啡酸甲酯(5)、新绿原酸(6)、绿原酸(7)、隐绿原酸(8)、阿魏酸(9)、烟酸(10)、1,2,4-苯三酚(11)、柠檬酸(12)、5,7-二羟基色原酮(13)、3,4-二咖啡酰奎宁酸(14)、新绿原酸内酯(15).其中,化合物11、化合物13及化合物15为首次从咖啡豆中分离得到.

The chemical constituents of the ethanol fraction of coffee beans were isolated and purified by HP-20 macroporous resin CC,semipreparative high performance liquid chromatography,and other separation methods.15 compounds were isolated from the ethanol fraction of coffee beans.By using NMR,MS,and other modern spectral techniques,the structures of the compounds were identified as caffeine(1),1,4-di-O-(E)-caffeoyl-β-D-glucopyranoside(2),1,3-O-dicaffeoylglycerol(3),caffeic acid(4),methyl caffeate(5),neochlorogenic acid(6),chlorogenic acid(7),4-caffeoylquinic acid(8),ferulic acid(9),nicotinic acid(10),1,2,4-benzenetriol(11),citric acid(12),5,7-dihydroxychromone(13),3,4-dicaffeoylquinic acid(14),and neochlorgeniclatone(15).Compounds 11,13 and compounds 15 were isolated from coffee beans for the first time.