藻蓝蛋白对博来霉素肺纤维化小鼠肠道菌群的影响

Effect of phycocyanin on the intestinal flora of mice with bleomycin-induced pulmonary fibrosis

博来霉素(bleomycin,BLM)是目前用于治疗头颈癌等多种癌症的药物之一,但会产生一系列副作用,例如肺纤维化(pulmonary fibrosis,PF),这大大降低了患者的生活质量。藻蓝蛋白(phycocyanin,PC)作为一种色素蛋白对包括PF在内的多种疾病具有调节作用。研究发现,慢性肠道疾病通常伴有肺部疾病,患者的肠道菌群会发生紊乱。针对上述线索,研究了PC对BLM诱导的PF小鼠肠道菌群的影响。通过尾静脉将BLM注入小鼠体内,以构建肺纤维化模型。然后通过16S r RNA高通量基因测序技术,分析了PC对BLM引起的PF小鼠肠道菌群的影响。结果表明,PC降低了BLM诱导的PF程度并调节了小鼠的肠道菌群。通过尾静脉注射BLM后,小鼠肺部受到损伤,肠道菌群发生紊乱。口服藻蓝蛋白干预后,小鼠的肺组织切片显示其胶原蛋白沉积减少,肺泡间隔和炎性细胞浸润减少,小鼠PF程度减轻。此时,小鼠肠道菌群中Alistipes、Lachnospiraceae等有益菌的丰度增加,Rikenella等有害菌的丰度降低,肠道菌群趋于正常。推测PC可以通过增加肠道中有益细菌的数量并减少肠道中有害细菌的数量来调节尾静脉注射BLM引起的肠道菌群失调,从而降低小鼠的PF程度。

Bleomycin(BLM)is currently used to treat various cancers,but it induces several side effects,such as pulmonary fibrosis(PF).Thus,the quality of life of patients is considerably reduced.Phycocyanin(PC),a pigment-protein complex,has been proven to be effective for many diseases,including PF.Studies have found that chronic intestinal diseases are usually accompanied by lung diseases,and the intestinal flora of patients is disordered.Based on the above findings,this article studied the effect of PC on intestinal flora in mice with BLM-induced PF.BLM was injected into mice through the tail vein to construct a model of PF.Combined with 16S rRNA high-throughput gene sequencing technology,the influence of PC on the intestinal flora of mice with BLM-induced PF was analyzed.Results showed that PC reduced the degree of PF induced by BLM and regulated the intestinal flora of mice.After mice were injected with BLM through the tail vein,lung injury was observed,and the intestinal flora was disturbed.Following oral PC intervention,lung tissue sections from mice showed the amelioration of PF,decreased collagen deposition,and reduced alveolar septum and inflammatory cell infiltration.At this time,the abundance of beneficial bacteria,such as Alistipes and Lachnospiraceae,was increased in the feces of mice,the abundance of harmful bacteria,such as Rikenella,decreased,and the intestinal flora tended to be normal.We speculate that PC can resolve the intestinal flora disorder caused by intravenous BLM injection by increasing the abundance of beneficial bacteria and reducing the abundance of harmful bacteria in the intestines,thereby reducing the degree of PF in mice.