UPLC-PDA切换波长法测定柴胡-白芍药对水煎液7种主要成分的含量

Determination of seven main components in aqueous extract of Bupleuri Radix-Paeoniae Alba Radix by UPLC-PDA

目的建立超高效液相色谱-二极管阵列检测器(UPLC-PDA)切换波长法同时测定柴胡-白芍药对水煎液中7种主要成分没食子酸、芍药内酯苷、芍药苷、苯甲酰芍药苷、柴胡皂苷A、柴胡皂苷C和柴胡皂苷D含量的方法。方法采用Waters ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱,流动相:乙腈-0.1%乙酸水溶液,梯度洗脱;流速为0.2 mL·min^(-1);检测波长分别为210(柴胡皂苷A、C、D)、237(芍药内酯苷、芍药苷、苯甲酰芍药苷)、278 nm(没食子酸),柱温30℃;进样量8μL。结果没食子酸、芍药内酯苷、芍药苷、苯甲酰芍药苷、柴胡皂苷A、柴胡皂苷C和柴胡皂苷D的线性范围分别为0.018~0.18μg(r=0.9999)、0.047~0.47μg(r=1)、0.52~5.18μg(r=0.9999)、0.021~0.21μg(r=0.9999)、0.043~0.43μg(r=0.9999)、0.073~0.73μg(r=0.9999)、0.042~0.42μg(r=0.9996),平均加样回收率(n=9)均在99.97%~101.47%,相对标准偏差(RSD)均小于2%。结论所建立的多成分含量测定方法快捷、准确、重复性好,可用于柴胡-白芍药对水煎液的质量控制。

Objective To establish a method for simultaneous determination of gallic acid,paeoniflorin,albiflorin,benzoyl paeoniflorin,saikosaponin A,saikosaponin C and saikosaponin D in the aqueous extract of Bupleurum and Paeoniae Alba by UPLC-PDA.Methods The Waters ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)column was used with the mobile phase composed of acetonitrile-0.1%acetic acid aqueous solution under gradient elution.The flow rate was 0.2 mL·min^(-1) at detection wavelengths of 210 nm for saikosaponin A,saikosaponin C and saikosaponin D,237 nm for paeoniflorin,albiflorin and benzoyl paeoniflorin,and 278 nm for gallic acid.The column temperature was 30℃while the injection volume was 8μL.Results The linear ranges of gallic acid,paeoniflorin,albiflorin,benzoyl paeoniflorin,saikosaponin A,saikosaponin C and saikosaponin D were 0.018~0.18μg(r=0.9999),0.047~0.47μg(r=1),0.52~5.18μg(r=0.9999),0.021~0.21μg(r=0.9999),0.043~0.43μg(r=0.9999),0.073~0.73μg(r=0.9999),and 0.042~0.42μg(r=0.9996)respectively.The average recovery(n=9)ranged from 99.94 to 101.47%,and the RSD was less than 2%.Conclusion This method is rapid,accurate and reproducible.It can be used for quality control of decoction of Radix Bupleuri-Paeoniae Alba.