摘要
The aim of this article was to investigate the influence and the related mechanism of the Retn gene on glucose uptake and insulin resistance in 3T3-L1 cells.Radioimmunoassay was used to determine glucose uptake in 3T3-L1 cells with different Retn gene expression levels,whether cells were stimulated by insulin or not.RT-PCR and real-time RT-PCR analysis were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different Retn gene expression levels,including insulin receptor substrate-1(IRS-1),phosphatidylinositol 3-kinase(PI-3K),AKT-2,glucose transporter-4(GLUT-4),p38 mitogen-activated protein kinase(p38MAPK)and glycogen synthase kinase-3b(GSK-3b).The glucose uptake decreased with the increase in Retn gene expression in 3T3-L1 cells,which was independent of whether the cells were stimulated by insulin or not.The mRNA expression of two signal proteins PI-3K and AKT-2 decreased and the other two signal proteins,GSK-3b and p38MAPK,increased with Retn overexpression in 3T3-L1 cells.Resistin could induce insulin resistance in adipocytes,which might be related to the changes of some proteins in PI-3K and Ras pathways.
基金
The study was supported by the National Natural Science Foundation of China(Grant No.30370802).
