Investigation of gene therapy of adenovirus in immune suppression

The aim of this paper is to investigate the safety of reconstructed adenovirus in immunosuppressive ther-apeutics and to explore the role of ciclosporin A in ant-agonizing the elimination of the vector.Several rats were given retroperitoneal injection of purified ADV-TK in order to obtain models.After 14 days’treatment of ciclos-porin A,samples of different periods were obtained,then stained with hematoxylin-eosin(HE)to detect inflam-mation reactions.Immunohistochemistry was used to examine the expression of adenovirus in organs.The results are as follows:(1)In HE stained sections of the organs,some transitory and reversible inflammation was detected.(2)In immunohistochemistry assay,recon-structed adenovirus decreased gradually as time went by in the control group,while it did not happen in the experi-mental group in which the adenovirus showed a relative increase compared with their counterparts(P,0.05).(3)The distributions of adenovirus in the liver,spleen and lung were higher than those in the other organs detected.Reconstructed adenovirus as a vector is definitely safe in immunosuppressive therapeutics,and ciclosporin A,to some extent,is able to consequently inhibit the immune response of the rats and prolong the existing period of adenovirus.