摘要
To study the effects of early signal substances induced by heat stress in brains of Kunming mice,six-month-old mice(n 572)were pretreated with heat stress and subsequent ischemia/reperfusion by clipping of their bilateral cervical common arteries for 7 min.According to different treatments,animals were randomly divided into four groups:(1)normal control group;(2)heat stress pre-treatment followed by ischemia and reperfusion group(HS/IR);(3)ischemia and reperfusion group(IR);(4)heat stress group(HS).Animals in the later three groups were subdivided into 3 subgroups(1 day,4 days,14 days),respectively.The changes in the expression of cAMP res-ponse element binding protein(CREB)and calcitonin gene-related peptide(CGRP)were detected by immuno-histochemistry and computer image analysis methods.The results showed that compared with the normal group,the expressions of CREB in the hippocampal CA1 region increased significantly in the HS,HS/IR and IR groups(P,0.05).Compared to the normal group,heat stress could result in CGRP excretion and redistribution in the cerebrum,with the highest level in the 4 d HS/IR group.Following heat stress,CGRP immunoreactivity was observed in varicose fibers and neuronal perikarya within the CA1 region.The results indicate that heat stress can induce CREB expression,which in turn stimulates CGRP secretion.
