基于非靶向代谢组学解析肉桂精油纳米乳抑制假单胞菌CM2作用机制研究

Antibacterial Mechanism of Cinnamon Essential Oil Nanoemulsion against Pseudomonas deceptionensis CM2 Based on Non-targeted Metabolomics

目的:利用非靶向代谢组学技术研究肉桂精油纳米乳(cinnamon essential oil nanoemulsion,CON)抑制假单胞菌CM2(Pseudomonas deceptionensis CM2)的作用机制。方法:P.deceptionensis CM2经CON处理4 h后,采用液相色谱-质谱分析技术鉴定差异代谢物,并分析差异代谢物的数量、种类及其在代谢通路中的调控表达。结果:采用二倍稀释法测得CON对P.deceptionensis CM2的最小抑菌浓度(minimal inhibitory concentration,MIC)为0.125μL/mL。多元统计分析表明,经1×MIC的CON处理4 h后,P.deceptionensis CM2的代谢特征存在显著差异;P.deceptionensis CM2细胞共出现380种差异代谢物,主要包括杂环化合物、芳香族化合物、脂类、有机酸等,其中309种差异代谢物含量显著升高(P<0.05),71种差异代谢物含量显著下降(P<0.05);这些差异代谢产物共参与13条代谢通路,主要涉及氨基酸、嘌呤、氨基糖、嘧啶等物质的代谢。结论:CON抑制P.deceptionensis CM2可能与其抑制氨基酸合成和能量代谢等代谢通路有关。本研究为CON在食品保鲜领域的应用提供了理论依据。

Objective:To investigate the underlying mechanism of cinnamon essential oil nanoemulsion(CON)-induced inactivation of Pseudomonas deceptionensis CM2 with the non-targeted metabolomics approaches.Methods:P.deceptionensis CM2 was treated with CON for 4 h,and the differential metabolites were identified by liquid chromatography-mass spectrometry combined.The number and type of differential compounds and their regulatory expression in metabolic pathways were also analyzed.Results:The minimum inhibitory concentration(MIC)of CON against P.deceptionensis CM2 was 0.125μL/mL.Multivariate statistical analysis showed that there were significant differences in the metabolic profiles of P.deceptionensis CM2 after exposure to CON at 1×MIC for 4 h.For P.deceptionensis CM2 cells treated with CON at 1×MIC for 4 h,a total of 380 differential metabolites were identified,mainly including heterocyclic compounds,aromatic compounds,lipids,organic acids,etc.Among these differential metabolites,309 metabolites were significantly up-regulated(P<0.05),and 71 metabolites were significantly down-regulated(P<0.05).These differential metabolites were mapped onto 13 metabolic pathways,mainly including the metabolism of amino acids,purine,amino sugar,pyrimidine,etc.Conclusion:CON might inactivate P.deceptionensis CM2 by affecting some metabolic pathways,such as amino acids biosynthesis and energy metabolism.This study would provide a theoretical basis for the application of CON in food preservation.