响应面法优化天然低共熔溶剂提取米糠蛋白工艺

Optimization of Rice Bran Protein Extraction Processing by Response Surface Methodology with Natural Deep Eutectic Solvents

本研究采用天然低共熔溶剂(Natural deep eutectic solvents,NADES)提取米糠蛋白的工艺并利用响应面方法对工艺参数进行优化,且通过采用傅里叶红外光谱和投射扫描电镜分析对天然低共熔溶剂提取米糠蛋白进行表征。结果表明,米糠蛋白提取最佳工艺条件为:含有4.7%水分的脯氨酸-甘油(摩尔比2:5)溶剂体系中,米糠和溶剂料液比为9:30 g/g,水浴搅拌3.0 h,反应温度为65℃,此时米糠蛋白提取率为82.69%,通过采用傅里叶红外光谱分析发现提取米糠蛋白过程中没有生成新物质,且天然低共熔溶剂能有效破坏米糠组织结构,释放米糠蛋白,从而提高米糠蛋白提取率。本研究为天然低共熔溶剂提取植物蛋白提供一定参考依据。

In this text,rice bran protein(RBP)was extracted from rice bran using natural deep eutectic solvents(NADES),as well as the extraction processing of parameters were systemically optimized.RBP was characterized by fourier transform infrared spectroscopy(FTIR)and scanning electron microscopy(SEM).Results showed that,the optimization extraction of RBP parameters were carried out as follows:Proline-glycerol(molar ratio 2:5),4.7% of water in system,solid-liquid ratio of 9:30 g/g for rice bran to NADES,reaction temperature at 65℃ for 3.0 h.The maximum extraction efficiency of RBP was 82.69% under the aforementioned conditions.The new chemical compound in the extraction processing of RBP were not able to be observed in terms of FTIR.The micrograph structure of rice bran was effectively damaged as shown in SEM,which might be a reasonable answer for the better extraction efficiency of RBP in NADES.The results in the present work guided for extraction of plant-based proteins with a designed NADES。