西洋参低聚糖的制备及其体外功能活性分析

Preparation and in Vitro Functional Activities of American Ginseng Oligosaccharides

通过酶法水解西洋参多糖(American ginseng polysaccharides,AGP)制得西洋参低聚糖(American ginseng oligosaccharides,AGOS),测定AGP和AGOS的ABTS+、DPPH、OH、O2-自由基清除率、Fe2+螯合能力和总还原力,α-葡萄糖苷酶和α-淀粉酶抑制能力,胆固醇结合能力、胆酸钠结合能力和脂肪酶抑制能力等,研究了AGP和AGOS的抗氧化活性、体外降血糖能力和体外降血脂能力。结果表明,纤维素酶酶解西洋参多糖的最佳条件为:反应温度45℃、底物浓度20 mg/mL、加酶量100 U/mg、酶解时间90 min,此条件下AGP酶解产物平均聚合度为2.74。西洋参低聚糖对ABTS+、DPPH和OH自由基具有更好的清除能力,其清除率分别达到了74.63%、79.98%和99.94%;对α-葡萄糖苷酶、α-淀粉酶和脂肪酶具有更好的抑制能力,其抑制率分别达到了35.76%、62.45%和98.55%。研究结果表明西洋参低聚糖较西洋参多糖具有更强的体外功能活性,可以作为具有潜力的功能性食品被进一步开发利用。

The American ginseng oligosaccharides(AGOS)were prepared by enzymatic hydrolysis of American ginseng polysaccharides(AGP).The antioxidant activity,in vitro hypoglycemic capacity and in vitro hypolipidemic capacity of AGP and AGOS were investigated by measuring their scavenging abilities on ABTS+,DPPH,OH,and O2-.The Fe2+chelating capacity,total reducing power,α-glucosidase andα-amylase inhibiting ability,cholesterol binding capacity,sodium cholate binding capacity and lipase inhibition capacity were also measured.The results showed that the optimal conditions for enzymatic hydrolysis of American ginseng polysaccharides by cellulase were:Reaction temperature of 45℃,substrate concentration of 20 mg/mL,cellulase dosage of 100 U/mg,and enzymolysis time of 90 min.The average polymerization degree of AGP enzymatic hydrolysate under the condition was 2.74.American ginseng oligosaccharides showed better scavenging ability on ABTS+,DPPH and OH,and the scavenging rates were 74.63%,79.98%and 99.94%,respectively,and had better inhibitory ability onα-glucosidase,α-amylase and lipase,the inhibition rates were 35.76%,62.45%and 98.55%,respectively.The results would indicate that American ginseng oligosaccharide had stronger in vitro functional activity than American ginseng polysaccharide,and could be further developed and utilized as potential functional foods.