FDFT1基因的泛癌表达分析、与癌症预后的关联性分析及其对几种肿瘤细胞增殖的影响

The analysis of pan-cancer FDFT1 gene expression with cancer prognosis,and its impact on the proliferation of various tumor cells

目的探究FDFT1(farnesyl-diphosphate farnesyltransferase 1)基因在不同肿瘤中的表达差异及其对肿瘤预后与肿瘤微环境的影响,及FDFT1过表达对几种肿瘤细胞增殖的影响。方法利用TCGA联合GTEx数据库分析FDFT1 mRNA在不同肿瘤组织与正常组织之间的表达差异;绘制K-M生存曲线,评估FDFT1基因表达与癌症预后的关系;利用细胞增殖实验探索FDFT1过表达对几种肿瘤细胞增殖的影响。结果与正常组织相比,在肾上腺皮质癌(adrenocortical carcinoma,ACC)等16种肿瘤组织中FDFT1 mRNA表达显著上调;在肾透明细胞癌(kidney renal cell carcinoma,KIRC)等8种肿瘤组织中FDFT1 mRNA表达下调。癌症预后相关性分析显示,FDFT1的高表达在ACC、胰腺癌(pancreatic adenocarcinoma,PAAD)、口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)及肝细胞癌(liver hepatocellular carcinoma,LIHC)中有较差的预后意义;而在KIRC中FDFT1的高表达与较好的预后有关。免疫浸润分析结果显示,与FDFT1基因低表达组相比,高表达组的ACC、OSCC、KIRC组织中活化的树突状细胞(activated dendritic cell,aDC)浸润评分较低;而在PAAD癌组织中,aDC浸润评分差异无统计学意义。细胞增殖实验结果显示FDFT1过表达促进人胰腺癌细胞(PANC-1)等细胞的增殖。结论FDFT1基因在ACC等16种肿瘤组织中mRNA表达上调,在KIRC等8种肿瘤组织中表达下调。FDFT1高表达在PAAD、OSCC和LIHC中与较差的预后有关,在KIRC中与较好的预后有关。FDFT1过表达慢病毒促进PANC-1等细胞的增殖。

Objective To investigate the differential expression of farnesyl-diphosphate farnesyltransferase 1(FDFT1)gene in different tumors and its impact on the tumor prognosis and microenvironment.Furthermore,to examine the effect of FDFT1 overexpression on the proliferation of various tumor cells.Methods This study analyzed the differential expression of FDFT1 mRNA in different tumor tissues and normal tissues using TCGA combined with GTEx databases.Kaplan-Meier(K-M)survival curves was plotted to evaluate the association between FDFT1 gene expression and cancer prognosis.Cell proliferation assays were conducted to explore the impact of FDFT1 overexpression on the proliferation of various tumor cells.Results Compared to normal tissues,the FDFT1 mRNA expression was significantly upregulated in 16 types of tumor tissues,including adrenocortical carcinoma(ACC),the FDFT1 mRNA expression was downregulated in 8 types of tumor tissues,including kidney renal cell carcinoma(KIRC).Correlation analysis of cancer prognosis revealed that high expression of FDFT1 had a poor prognosis in ACC,PAAD,oral squamous cell carcinoma(OSCC)and liver hepatocellular carcinoma(LIHC).In contrast,high expression of FDFT1 was associated with better prognosis in KIRC.Immune infiltration analysis showed that,the activated dendritic cell(aDC)infiltration scores were lower in ACC,OSCC and KIRC tissues with high-expression compared to the low-expression group of FDFT1 gene.However,there was no statistically difference in aDC infiltration scores in PAAD tissues.The results of the cell proliferation assay showed that FDFT1 overexpression promoted the proliferation of cells including human pancreatic cancer(PANC-1)cells.Conclusion The FDFT1 gene was up-regulated in mRNA expression of 16 tumor tissues including ACC,and it was downregulated in 8 tumor tissues including KIRC.High expression of FDFT1 was associated with poor prognosis in PAAD,OSCC and LIHC,while high expression of FDFT1 was associated with better prognosis in KIRC.FDFT1 overexpression lentivirus promotes the proliferation of cells including PANC-1 cells.