基于生物信息学分析揭示枸杞红素抗青光眼靶点及分子机制的研究

Study on the anti-glaucoma target and molecular mechanism ofZeaxanthin dipalmitate based on bioinformatics analysis

目的本研究拟通过网络药理学研究策略旨在揭示枸杞红素抗青光眼的药理靶点及分子机制。方法运用整合数据库分别获取枸杞红素治疗靶点和青光眼病理靶点,并经映射后获得枸杞红素治疗青光眼的潜在靶点。采用STRING数据库获得靶点-靶点功能相关蛋白间的网络关联图。运用Cytoscape软件Network Analyzer分析网络自由度中位数和最大自由度等拓扑参数,并根据Degree值筛选现枸杞红素抗青光眼的核心靶点。此外,应用R语言中的“ClusterProfiler”、“org.Hs.eg.Db”、“ggplot2”等数据包对核心靶点进行GO和KEGG富集分析及可视化。结果生物信息学分析筛选出128个枸杞红素、1196个青光眼潜在靶点及19个共有靶点。GO富集分析结果表明枸杞红素抗青光眼药理活性可能与通过调控这些核心靶点有关,提示枸杞红素通过多靶点调控进而发挥治疗青光眼的潜在作用。KEGG富集分析结果显示,枸杞红素抗青光眼作用可能与调控眼组织细胞因子活性,内环境稳态及激活神经修复因子/受体活性进而促进神经细胞增殖或修复有关。此外,枸杞红素抗青光眼分子机制可能与抗炎症反应、氧化损伤、细胞凋亡及增强免疫调控等信号通路有关。结论枸杞红素可能是抗青光眼的潜在活性成分,并通过多靶点和多通路共同作用发挥抗青光眼活性。

Objective This study aims to reveal pharmacological targets and molecular mechanisms of Zeaxanthin dipalmitate in the treatment of glaucoma through network pharmacological research strategies.Methods Integrated databases were utilized to obtain the therapeutic targets of Zeaxanthin dipalmitate and the pathological targets of glaucoma.Mapping techniques were employed to identify potential targets of Zeaxanthin dipalmitate for glaucoma treatment.The STRING database was used to construct a network correlation graph between the targets and function-related proteins.Network Analyzer was utilized to analyze topological parameters,such as median and maximum degrees of freedom,and the core target of antiglaucoma of Zeaxanthin dipalmitatewas selected according to the Degree value.In addition,GO and KEGG enrichment analysis and visualization of the core targets were performed using R language packages,including ClusterProfiler,org.Hs.eg.Db,and ggplot2.Results Bioinformatics analysis identified 128 potential targets of Zeaxanthin dipalmitate,1196 potential targets of glaucoma,and 19 common targets.The results of GO enrichment analysis showed that the anti-glaucoma pharmacological activity of Zeaxanthin dipalmitate may be related to the regulation of these core targets,suggesting its potential role in the treatment of glaucoma through multi-target regulation.KEGG enrichment analysis showed that the anti-glaucoma effect of Zeaxanthin dipalmitate may be related to the regulation of ocular tissue cytokine activity,homeostasis of internal environment and activation of nerve repair factor/receptor activity to promote the proliferation or repair of nerve cells.In addition,the molecular mechanism of Zeaxanthin dipalmitate against glaucoma may be related to the signaling pathways of anti-inflammation,oxidative damage,cell apoptosis and enhanced immune regulation.Conclusion Zeaxanthin dipalmitate may serve as a potential active ingredient in the treatment of glaucoma,exerting its anti-glaucoma activity through multi-target and multi-pathway interactions.