不同花色类型蜡梅花被片靶向类黄酮代谢组学分析

Metabonomic Analysis of Targeted Flavonoids for Tepals of Different Flower Color Types of Wintersweet

[目的]以初花期的红花蜡梅、红心蜡梅、素心蜡梅的中、内花被片为材料,开展靶向类黄酮代谢组检测与分析,为进一步解析不同花色类型蜡梅花被片的呈色物质及类黄酮代谢途径提供参考。[方法]对蜡梅3个品种的中、内花被片进行UPLC-MS/MS检测,并进行代谢物定性定量分析、样本质控分析、PCA分析、HCA分析、OPLS-DA分析及差异代谢物KEGG功能注释与富集分析,探究不同花色类型花被片间类黄酮代谢物差异。[结果]在蜡梅花被片中共检测到82种代谢物,包括查耳酮、二氢黄酮、黄酮、异黄酮、二氢黄酮醇、黄酮醇、花青苷、黄烷醇等化合物;蜡梅红色花被片类黄酮物质除花青苷类外,还包括黄酮醇类化合物,其在红、黄花被片中未显示出规律性变化;花青素代谢通路富集程度在蜡梅红、黄花被片5个比较组中均表现为最大且最显著。[结论]蜡梅花被片类黄酮生物合成途径中除主要的黄酮醇支路和花青素支路外,还可能包括黄酮支路、异黄酮支路和黄烷醇支路;在蜡梅花被片类黄酮生物合成途径中,花青素支路和黄酮醇支路可能不是对共同底物的竞争关系;矢车菊苷是蜡梅花被片呈红色的特征代谢物,包括矢车菊素-3-O-葡萄糖苷、矢车菊素-3-O-芸香糖苷和矢车菊素-3-O-半乳糖苷。

[Objective]Targeted flavonoid metabolome detection and analysis were carried out to provide reference for further analysis of color substances and flavonoid metabolism pathway in the tepals of different flower color types of wintersweet using the middle and inner tepals of three cultivars of Rubrum wintersweet,Patens wintersweet,and Concolor wintersweet at early flowering stage.[Methods]UPLC-MS/MS was used to detect the middle and inner tepals of three wintersweet cultivars,and the qualitative and quantitative analysis of metabolites,sample quality control analysis,PCA,HCA,OPLS-DA,KEGG functional annotation and enrichment analysis of differential metabolites were used to explore the differences of flavonoid metabolites in the tepals of different flower color types.[Results]A total of 82 metabolites were detected in wintersweet tepals,including chalcone,dihydroflavone,flavone,isoflavone,dihydroflavonol,xanthone alcohol,anthocyanin,flavanol;In addition to anthocyanins,the flavonoids in the red tepal of wintersweet also included flavonol compounds,which did not show regular changes in the red and yellow tepals;In the five comparison groups of wintersweet red tepals and yellow tepals,the enrichment of anthocyanin metabolic pathway was the largest and most significant.[Conclusion]Besides the main flavonol branch and anthocyanin branch,the flavone branch,isoflavone branch,and procyanidin branch may also be included in the flavonoid biosynthesis pathway of wintersweet tepals;The anthocyanin branch and flavonol branch may not compete for the common substrate in the flavonoid biosynthesis pathway of the red tepals of wintersweet;Cyanidin glycosides are the characteristic metabolites of the red tepals in wintersweet,including cyanidin 3-O-glucoside,cyanidin 3-O-rutinoside,and cyanidin 3-O-galactoside.