摘要
目的:探讨低增生性骨髓增生异常综合征(hypo-MDS)和再生障碍性贫血(AA)外周血淋巴细胞亚群及其他临床指标的差异。方法:应用流式细胞术检测hypo-MDS(n=25)和AA(n=33)患者的淋巴细胞亚群,同时分析两组间外周血象、生化指标、骨髓各细胞组分以及异常染色体的差异。结果:AA组CD8^(+)T细胞比例明显高于hypo-MDS组(P=0.001),而CD4^(+)T细胞比例和CD4^(+)/CD8^(+)比率明显低于hypo-MDS组(P=0.015;P=0.001)。与hypo-MDS组相比,AA组CD4^(+)、CD8^(+)活化T(TA)细胞及记忆Treg比例均明显下降(P=0.043;P=0.015;P=0.024),而AA组CD8^(+)初始T(TN)细胞比例较高(P=0.044)。与AA组相比,Hypo-MDS组外周血淋巴细胞数明显降低(P=0.025),总胆红素(TBil)、乳酸脱氢酶(LDH)、维生素B12水平及骨髓原始细胞比例均明显升高(P=0.019;P=0.023;P=0.027;P=0.045)。结论:AA组和hypo-MDS组CD4^(+)和CD8^(+)TA细胞、记忆Treg细胞及CD8^(+)TN细胞比例差异明显,可为鉴别这两种疾病提供重要依据。
Objective:To explore the difference of lymphocyte subsets in peripheral blood(PB)between aplastic anemia(AA)and hypoplastic myelodysplastic syndrome(hypo-MDS)patients,meanwhile to compare the clinical parameters obtained from PB and bone marrow(BM).Methods:The lymphocyte subsets in hypo-MDS(n=25)and.AA(n=33)patients were investigated by flow cytometry.Meanwhile,the differences in PB cell counts,biochemical indicators,BM cell counts and abnormal chromosomes between the two groups were analyzed.Results:The percentage of CD8^(+)T cells in AA group was significantly higher than that in hypo-MDS group(P=0.001),while the percentage of CD4^(+)T cells and the CD4^(+)/CD8^(+)ratio in AA group were obviously lower than those in hypo-MDS group(P=0.015 and 0.001,respectively).Furthermore,the proportion of CD4^(+)and CD8^(+)activated T(TA)cells,and memory Tregs in AA group was distinctly lower than those in hypo-MDS group(P=0.043,0.015 and 0.024,respectively).Nevertheless,the percentage of CD8^(+)naive T(TN)cells in AA patients was remarkably higher(P=0.044).And hypo-MDS patients had declined lymphocyte counts(P=0.025),increased levels of total bilirubin(TBil),lactate dehydrogenase(LDH),vitamin B12 and proportion of BM blasts than AA patients(P=0.019,0.023,0.027 and.0.045,respectively).Conclusion:In this study it was confirmed that the percentages of CD4^(+)and CD8^(+)TA cells,memory Tregs and CD8^(+)TN cells were significantly different between AA and hypo-MDS patients,which provide an essential basis for the identification of these two diseases.
作者
吴鸿飞
王世充
黄金波
霍佳莉
邵英起
任翔
李星鑫
王敏
聂能
张静
金朋
葛美丽
郑以州
WU Hong-Fei;WANG Shi-Chong;HUANG Jin-Bo;HUO Jia-Li;SHAO Ying-Qi;REN Xiang;LI Xing-Xin;WANG Min;NIE Neng;ZHANG Jing;JIN Peng;GE Mei-Li;ZHENG Yi-Zhou(State Key Laboratory of Experimental Hematology,National Clinical Research Center for Blood Diseases,Haihe Laboratory of Cell Ecosystem,Institute of Hematology&Blood Diseases Hospital,Chinese Academy of Medical Science&Peking Union Medical College,Tianjin 300020,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2023年第4期1125-1132,共8页
Journal of Experimental Hematology
基金
国家自然科学基金面上项目(81770119)
中国医学科学院基金(2021-RW320-011)。