GC法同时测定壮骨麝香止痛凝胶贴膏中5种挥发性成分

Simultaneous determination of five volatile constituents in Zhuanggu Shexiang Zhitong Gel Paste

目的建立GC法同时测定壮骨麝香止痛凝胶贴膏中薄荷脑、樟脑、龙脑、异龙脑、水杨酸甲酯的含量。方法该药物乙酸乙酯提取液的分析采用DB-WAX色谱柱(30 m×0.25 mm×0.50μm);载气氮气;FID检测器,温度240℃;分流比5∶1;程序升温。结果5种挥发性成分在各自范围内线性关系良好(R^(2)≥0.9997),平均加样回收率97.40%~99.88%,RSD 1.6%~4.0%。结论该方法准确、稳定、可靠,可用于壮骨麝香止痛凝胶贴膏的质量控制。

AIM To establish a GC method for the simultaneous content determination of L-menthol,camphor,borneol,isoborneol and methyl salicylate in Zhuanggu Shexiang Zhitong Gel Paste.METHODS The analysis of ethyl acetate extract of this drug was performed on DB-WAX column(30 m×0.25 mm×0.50μm),the carrier gas was nitrogen,FID detector temperature was 240℃,and the split ratio was 5∶1 in temperature programming.RESULTS Five volatile constituents showed good linear relationships within their own ranges(R^(2)≥0.9997),whose average recoveries were 97.40%-99.88%with the RSDs of 1.6%-4.0%.CONCLUSION This accurate,stable and reliable method can be used for the quality control of Zhuanggu Shexiang Zhitong Gel Paste.