Advances in plant proteomics—key techniques of proteome

Following the completion of genome sequencing of model plants,such as rice(Oryza sativa L.)and Arabidopsis thaliana,the era of functional plant genomics has arrived which provides a solid basis for the develop-ment of plant proteomics.We review the background and concepts of proteomics,as well as the key techniques which include:(1)separation techniques such as 2-DE(two-dimensional electrophoresis),RP-HPLC(reverse phase high performance liquid chromatography)and SELDI(surface enhanced laser desorption/ionization)protein chip;(2)mass spectrometry such as MALDI-TOF-MS(matrix assisted laser desorption/ionization-time of flight-mass spectrometry)and ESI-MS/MS(electrospray ionization mass spectrometry/mass spectrometry);(3)Peptide sequence tags;(4)databases related to proteomics;(5)quantitative proteome;(6)TAP(tandem affinity purification)and(7)yeast two-hybrid system.In addition,the challenges and prospects of pro-teomics are also discussed.