响应面法优化禾谷镰刀菌产脱氧雪腐镰刀菌烯醇及其衍生物的液体培养条件

Optimization of Liquid Culture Conditions for the Production of Deoxynivalenol and Its Derivatives by Fusarium graminearum Using Response Surface Methodology

为研究不同液体培养条件对禾谷镰刀菌脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)和15-乙酰基脱氧雪腐镰刀菌烯醇(15-acetyldeoxynivalenol,15-ADON)产量的影响,采用单因素试验和响应面法进行研究。结果表明,培养时间、pH及两者间交互作用对DON产量影响极显著;培养时间及培养时间与培养温度的交互作用对15-ADON产量影响极显著。DON的最佳产毒条件为:培养温度26.3℃,初始pH 6.35,培养22 d,此条件下DON的产量达5739.69μg·L^(-1);15-ADON的最佳产毒条件为:培养温度26.3℃,初始pH 5.58,培养21 d,此条件下15-ADON的产量达10453.41μg·L^(-1),为DON及15-ADON的生物合成、日常监管和污染防控等提供理论依据和数据支持。

To investigate the effects of different liquid culture conditions on the production of deoxynivalenol(DON)and 15-acetyldeoxynivalenol(15-ADON)by Fusarium graminearum,the single factor experiment and response surface methodology were used.The results showed that the production of DON was significantly influenced by incubation time,initial pH and their interactive effect,while the production of 15-ADON was significantly influenced by incubation time,incubation temperature and their interactive effect.The optimal conditions for DON production was followed with incubation time 22 d,temperature 26.3℃,initial pH 6.35,which the highest amount of DON was 5739.69μg·L^(-1).The highest production of 15-ADON with 10453.41μg·L^(-1) was achieved when the incubation time,the temperature and the initial pH were set as 21 d,26.3℃and 5.58,respectively.The above results provided theoretical basis and data support for the biosynthesis,routine supervision and control of DON and 15-ADON.