摘要
We designed specific small interfering RNA(siRNA)targeting vascular endothelial growth factor(VEGF)mRNA and synthesized oligo fragments,then siRNA was obtained by in vitro transcription and transfected into cultured human colon carcinoma cell line HT-29 with lipofectamine.We also analyzed the effect of the siRNA on proliferation of HT-29 cells by methyl thiazolyl tetrazolium(MTT)assay and expression level of VEGF mRNA of transfected cells by RT-PCR as well as amounts of secreted VEGF protein in the supernatant by enzyme linked immunosorbent assay(ELISA).Two groups of siRNA targeting human VEGF effectively inhibited proliferation of HT-29 cells after transfection.The secretion of VEGF protein also notably decreased,but the control scramble siRNA showed no effect.
基金
This work was supported by Qingdao Science and Technology Project(No.03-1-YN-14).
