扁刺蛾线粒体基因组全序列特征及系统发育分析

The Complete Mitochondrial Genome Sequence and Phylogenetic Analysis of Thosea sinensis

扁刺蛾(Thosea sinensis)具有分布广、多食性、危害大等特点,是我国重要的农林业害虫。为报道采自江西的扁刺蛾线粒体基因组,了解其线粒体基因组的多样性与差异,探究刺蛾科昆虫线粒体基因组进化规律。通过Sanger测序后拼接、校正、注释获得扁刺蛾的线粒体全基因组序列,并基于蛋白质编码基因序列构建了鳞翅目17个科26种蛾类昆虫的系统发育树。结果显示,扁刺蛾线粒体基因组是1个大小为15540 bp的闭合环状双链DNA分子,共编码37个基因,包括13个蛋白质编码基因,2个核糖体RNA基因和22个转运RNA基因,还有1个425 bp的控制区,基因排列与鳞翅目双孔类(Ditrysia)昆虫相同。通过与其他刺蛾的全序列和蛋白质编码基因序列对比相似度,结果显示,扁刺蛾与茶刺蛾(Iragoides fasciata)的相似度最高,与褐边绿刺蛾(Parasa consocia)相似度最低。系统发育分析结果表明,扁刺蛾与茶刺蛾的亲缘关系最近,其次为龟形小刺蛾(Narosa nigrisigna),鳞翅目昆虫各科均聚为一支。本研究为深入研究扁刺蛾的起源、遗传多样性、迁移和分化,以及对农药的抗性提供科学依据。

Thosea sinensis is an important agricultural and forestry pest in China with characteristics of wide distribution,polyphagy,and high damage.The purpose of this study was to report the mitochondrial genome of T.sinensis collected from Jiangxi,investigate its diversity and difference,and explore the evolutionary characteristics of Limacodidae insects.After Sanger sequencing,the complete mitochondrial genome sequence of T.sinensis was obtained by splicing,correcting and annotating,and the phylogenetic tree of 26 moth species in 17 families of Lepidoptera was constructed based on the protein sequences.The complete mitochondrial genome sequence was 15540 bp in size,encoding 37 genes,including 13 protein-coding genes,2 ribosomal RNAs,22 transfer RNA genes,and 1 control region of 425 bp.The gene arrangement is the same as that of the Ditrysia moths.By comparing the similarity of the full sequence and protein-coding genes of the mitochondrial genomes with other moths,the results show that the similarity between T.sinensis and Iragoides fasciata was the highest,and that between T.sinensis and Parasa consocia was the lowest.Phylogenetic analysis shows that the closest relationship of T.sinensis was with Iragoides fasciata,followed by Narosa nigrisigna,and all the moths from Lepidoptera were clustered into one branch.This study provided a scientific basis for further research on the origin,genetic diversity,migration,and differentiation of T.sinensis,as well as its resistance to pesticides.