阿克曼嗜黏液菌外膜蛋白1100促进糖尿病大鼠创面愈合

Akkermansia muciniphila outer membrane protein 1100 promotes wound healing in diabetic rats

目的探究阿克曼嗜黏液菌外膜蛋白1100(Amuc_1100)对糖尿病大鼠创面炎症及胶原纤维合成的影响。方法20只雄性SD大鼠腹腔注射链脲佐菌素诱导为糖尿病大鼠,构建背部全层皮肤缺损创面模型并分为2组:对照组及Amuc_1100组。Amuc_1100组在创面局部多点注射300μL浓度为333μg/mL的Amuc_1100溶液,对照组注射等量生理盐水。第0、3、7、10、14天创面拍照比较创面愈合率。第7、14天每组各处死5只大鼠并创面取材,HE染色评估创面炎症细胞浸润及组织学形态;Masson染色评估创面胶原纤维沉积情况;RT-PCR比较肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、胶原纤维1A1(Col1A1)、胶原纤维3A1(Col3A1)mRNA相对表达量。结果造模后第3、7、10、14天,Amuc_1100组创面愈合率均高于对照组(P<0.05);HE染色显示Amuc_1100组创面组织中炎症细胞聚集更少,胶原纤维沉积更多;Masson染色显示Amuc_1100组创面胶原更为粗大、深染,排列更为致密;RT-PCR显示Amuc_1100组创面内TNF-α、IL-6 mRNA相对表达较对照组减少(P<0.05),IL-10、Col1A1、Col3A1 mRNA相对表达水平较对照组增多(P<0.05)。结论Amuc_1100能够提升糖尿病大鼠创面愈合速率,其机制可能与降低局部炎症反应,增加胶原纤维合成有关。

Objective To investigate the effects of Akkermansia muciniphila outer membrane protein 1100(Amuc_1100)on wound inflammation and collagen fiber synthesis in diabetic rats.Methods Twenty male SD rats were induced into diabetic rats by intraperitoneal injection of streptozotocin.The defective wound model of full-thickness back skin was constructed and the rats were divided into two groups,control group and Amuc_1100 group.The Amuc_1100 group was injected locally with 300μL Amuc_1100 solution with a concentration of 333μg/mL,the control group was injected with the same amount of normal saline.Wounds were photographed and the wound healing rate were compared on day 0,3,7,10 and 14,respectively.Five rats in each group were sacrificed and harvested on day 7 and 14.Hematoxylin-eosin(HE)staining was used to evaluate inflammatory cell infiltration and histological morphology of the wound.Masson three-color staining was used to evaluate collagen fiber deposition.The mRNA expression levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-10(IL-10),collagen fiber 1A1(Col1A1)and collagen fiber 3A1(Col3A1)were compared by real-time quantitative polymerase chain reaction(RT-PCR).Results The wound healing rate in Amuc_1100 group was higher than that in control group on day 3,7,10 and 14(P<0.05).HE staining showed that the Amuc_1100 group had less inflammatory cells and more collagen fiber deposition in wound tissue.Masson three-color staining showed that the collagen in the Amuc_1100 group was thicker,deeper dyed and more compact than control group.RT-PCR showed that the relative mRNA expressions of TNF-αand IL-6 were significantly decreased in Amuc_1100 group compared with the control group(P<0.05),and the relative mRNA expressions of IL-10,Col1A1 and Col3A1 were significantly increased compared with the control group(P<0.05).Conclusion Amuc_1100 can improve the wound healing rate of diabetic rats,and the mechanism may be related to reducing local inflammatory response and increasing collagen fiber synthesis.