新疆小麦白粉病菌群体遗传多样性分析

Analysis of Genetic Diversity of Blumeria graminis f.sp.tritici Population in Xinjiang Province

为了解新疆小麦白粉病菌群体多样性及其间关系,利用ISSR分子标记对2020年喀什、阿克苏、和田、昌吉、塔城共5个地区13个县市小麦白粉病菌群体进行遗传多样性研究。结果表明,喀什、阿克苏、和田、昌吉、塔城小麦白粉病菌群体的遗传距离为0.0317~0.0945,塔城与阿克苏群体的遗传距离最近,和田与喀什的遗传距离最远;总体Nei s遗传多样性指数为0.2571,群体Nei s内遗传多样性指数为0.2175,群体内的遗传变异占总体变异的84.60%,表明遗传变异主要来源于群体内。群体间基因流N m值为2.7490,表明新疆不同区域群体存在一定的基因交流。Normalized Mantel Statistic分析表明,小麦白粉病菌群体间遗传距离与地理距离相关性不大。

In order to clarify population genetic diversity of the wheat powdery mildew pathogen in Xinjiang,simple repeat sequence intervals(ISSR)molecular markers were used to analyze population genetic diversity of the wheat powdery mildew pathogen isolates from 13 counties in five regions,including Kashgar,Aksu,Hotan,Changji,and Tacheng in 2020.The results showed that genetic distance of five populations of Kashgar,Aksu,Hotan,Changji,and Tacheng ranged from 0.0317 to 0.0945,among which genetic distance between Tacheng and Aksu populations was minimum,and that between Hotan and Kashgar was maximum.Genetic diversity index of overall population was 0.2571,and that within population was 0.2175.Genetic variation within population accounted for 84.60%of the total variations,indicating that the genetic variation mainly originated within the population.The value of gene flow(N m)between populations was 2.7490,indicating that there was a certain gene exchange among populations in different regions of Xinjiang.Normalized Mantel Statistic analysis showed that there was not a strong correlation of genetic distance and geographic distance between populations of the wheat powdery mildew pathogen.