猪Caskin1基因生物信息学分析

Bioinformatics analysis of porcine Caskin1 gene

为了分析猪钙/钙调素依赖性丝氨酸蛋白激酶(CASK)相互作用蛋白1(Caskin1)基因和编码蛋白的结构与功能,并探讨其在改善生殖衰老中的潜在可能性,试验首先获取猪Caskin1基因及其蛋白序列,采用生物信息学分析方法对Caskin1基因编码蛋白的理化性质、磷酸化位点、高级结构、相互作用网络和保守基序等进行研究,构建猪Caskin1基因系统发育树,分析该基因的组织表达谱。结果表明:猪Caskin1基因编码序列(CDS)长度为4359 bp,可编码1452个氨基酸;猪Caskin1蛋白的分子量为151782.89 u,理论等电点为9.21;其二级结构以无规则卷曲结构(61.16%)为主,为不稳定亲水性蛋白,不存在信号肽,没有跨膜结构;猪Caskin1基因为保守基因,其编码蛋白的SH3结构域是高度保守结构域,且该蛋白质与CASK、SYT7、NSF、CADPS、P14K2A、DPYSL4等多个蛋白质间存在相互作用;猪Caskin1基因与牛、羊的亲缘关系最近,与原鸡的亲缘关系最远;该基因不仅在大脑中高表达,在卵巢中的相对表达量也比较高。说明猪Caskin1基因及蛋白的生物学功能丰富,生物学意义较为重要。

The objective of this study was to analyse the structure and function of the porcine Caskin1 gene and its encoded protein,and to explore its potential function in disease treatment and improving reproductive ageing.In the present experiment,firstly,the porcine Caskin1 gene and its protein sequence were obtained.Then,bioinformatics analysis was used to predict the evolutionary tree of porcine Caskin1 gene,and the tissue expression profile and molecular structure of the gene were analyzed.At the same time,the physicochemical properties,phosphorylation sites,advanced structure,interaction network and conserved motifs of the encoded protein were studied.The results showed that the coding region of porcine Caskin1 gene was 4359 bp,encoding 1452 amino acids.The molecular weight of the protein was 151782.89 u and the theoretical isoelectric point was 9.21.The secondary structure of the protein was mainly irregular structure(61.16%).Porcine Caskin1 protein was an unstable hydrophilic protein without signal peptide or transmembrane structure.The porcine Caskin1 gene was conserved,and the SH3 domain of the protein encoded by Caskin1 was highly conserved,and the protein interacted with CASK,SYT7,NSF,CADPS,P14K2A,DPYSL4 and other proteins.Pigs were closest to cattle and sheep,and furthest to chickens.Caskin1 gene was not only highly expressed in the brain,but also in the ovaries.These results indicated that Caskin1 gene and protein had rich biological functions and important biological significance.