摘要
目的设计并构建一种氧化石墨烯(graphene oxide,GO)/磷酸银(Ag_(3)PO_(4))/壳聚糖(chitosan,CS)复合涂层,用于植入物手术中快速杀灭细菌,预防术后感染。方法使用离子交换法制备GO/Ag_(3)PO_(4)复合材料,并在生物钛(Ti)片上依次沉积CS和GO/Ag_(3)PO_(4)复合材料,制备Ti-GO/Ag_(3)PO_(4)/CS材料。对材料的形貌、物相、光热和光催化性能、抗菌能力以及与基体的结合力进行表征。结果使用离子交换法成功制备GO/Ag_(3)PO_(4)复合材料,通过形貌物相测试证明了GO/Ag_(3)PO_(4)的异质结构,Ag_(3)PO_(4)和GO形成的异质结构使带隙从1.79 eV降低到1.39 eV,可以被808 nm近红外光激发。光热、光催化实验表明GO/Ag_(3)PO_(4)/CS复合涂层具有良好的光热和光动力性能;体外抗菌实验表明,GO/Ag_(3)PO_(4)/CS复合涂层在808 nm近红外光照射20 min后对金黄色葡萄球菌的抑菌率达到99.81%;同时该复合涂层具有良好的光稳定性,可以提供稳定和持续的抗菌效果。结论GO/Ag_(3)PO_(4)/CS复合涂层可以被808 nm近红外光激发产生活性氧,在光照下具有优异的抗菌能力。
Objective To design and construct a graphene oxide(GO)/silver nitrate(Ag_(3)PO_(4))/chitosan(CS)composite coating for rapidly killing bacteria and preventing postoperative infection in implant surgery.Methods GO/Ag_(3)PO_(4) composites were prepared by ion exchange method,and CS and GO/Ag_(3)PO_(4) composites were deposited on medical titanium(Ti)sheets successively.The morphology,physical image,photothermal and photocatalytic ability,antibacterial ability,and adhesion to the matrix of the materials were characterized.Results The GO/Ag_(3)PO_(4) composites were successfully prepared by ion exchange method and the heterogeneous structure of GO/Ag_(3)PO_(4) was proved by morphology phase test.The heterogeneous structure formed by Ag_(3)PO_(4) and GO reduced the band gap from 1.79 eV to 1.39 eV which could be excited by 808 nm near-infrared light.The photothermal and photocatalytic experiments proved that the GO/Ag_(3)PO_(4)/CS coating had excellent photothermal and photodynamic properties.In vitro antibacterial experiments showed that the antibacterial rate of the GO/Ag_(3)PO_(4)/CS composite coating against Staphylococcus aureus reached 99.81%after 20 minutes irradiation with 808 nm near-infrared light.At the same time,the composite coating had excellent light stability,which could provide stable and sustained antibacterial effect.Conclusion GO/Ag_(3)PO_(4)/CS coating can be excited by 808 nm near infrared light to produce reactive oxygen species,which has excellent antibacterial activity under light.
作者
王轶凡
许英德
张雪枫
刘菁宇
韩金彤
朱胜利
梁砚琴
吴水林
崔振铎
吕维加
李朝阳
WANG Yifan;XU Yingde;ZHANG Xuefeng;LIU Jingyu;HAN Jintong;ZHU Shengli;LIANG Yanqin;WU Shuilin;CUI Zhenduo;LÜWeijia;LI Zhaoyang(Tianjin Key Laboratory of Composite and Functional Materials,School of Materials Science and Engineering,Tianjin University,Tianjin,300350,P.R.China;Faculty of Pharmaceutical Sciences,Shenzhen Institute of Advanced Technology Chinese Academy of Sciences,Shenzhen Guangdong,518055,P.R.China)
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2023年第8期937-944,共8页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目(51871163)
天津市自然科学基金资助项目(20JCZDJC00580)。
关键词
细菌感染
抗菌涂层
异质结构
光热-光动力抗菌
Bacterial infection
antibacterial coating
heterojunction
photothermal-photodynamic antibacterial
