基于宏基因组学分析海南省不同养殖场库蠓共生微生物群落组成

A metagenomic analysis of the composition of symbiotic microbial communities in Culicoides on different livestock farms in Hainan Province

目的探究海南省不同养殖场库蠓共生微生物群落多样性和差异性。方法2021年8-9月,在海南省文昌市、定安和乐东县5个不同的养殖场(牛圈、鸡舍、犬场、羊圈和猪圈),用紫外灯诱法采集库蠓,分别使用十六烷基三甲基溴化铵法提取总DNA后,通过宏基因组学测序技术分析其微生物群落结构特征及基因功能分布。结果不同养殖场库蠓优势种不同,牛圈、羊圈和猪圈主要是尖喙库蠓,占比分别为92.91%,82.22%和95.85%;鸡舍和犬场均以荒川库蠓为主要优势种,占比分别为66.36%和98.22%。不同养殖场库蠓共生微生物在门水平上,均以变形菌门(33.78%)、子囊菌门(9.09%)和厚壁菌门(8.88%)为主要菌门;在属水平上,均以不动杆菌属(22.48%)和梭菌属(7.25%)为优势菌属。属水平微生物多样性分析发现5个养殖场共有的属最多,有3357属。微生物基因差异分析显示5个不同的养殖场共有基因数目较少,为1065个。此外,功能预测结果也显示,29.10%注释基因与新陈代谢相关,如碳水化合物代谢、氨基酸代谢和脂质代谢等。基因数目差异分析结果表明,库蠓种类组成越相似,样品共有基因数目越多。结论微生物群落分析发现,海南省不同养殖场库蠓共生微生物的核心菌群相对稳定,库蠓优势种相同的养殖场微生物群落相似度更高。该研究丰富了我国库蠓的微生物群落数据库,对今后库蠓的生物防控有重要意义。

Objective To explore the diversity and variability of symbiotic microbial communities in Culicoides on different livestock farms in Hainan Province,China.Methods From August to September 2021,the ultraviolet lamptrap method was used to collect Culicoides on five different livestock farms(cattle farms,chicken houses,dog houses,sheep pens,and pigsties)in Wenchang City,Ding’an County,and Ledong County in Hainan Province.After the total DNA was extracted by hexadecyltrimethylammonium bromide method,the structural characteristics and functional distribution of genes in the microbial communities were analyzed using metagenomic sequencing.Results The dominant species of Culicoides on different livestock farms varied.The dominant species on cattle farms,sheep pens,and pigsties was C.oxystoma,accounting for 92.91%,82.22%,and 95.85%,respectively;the dominant species in chicken houses and dog houses were C.arakawae,accounting for 66.36%and 98.22%,respectively.On different livestock farms,Proteobacteria(33.78%),Ascomycota(9.09%),and Firmicutes(8.88%)were the dominant phyla of the symbiotic microbial communities in Culicoides,and Acinetobacter(22.48%)and Clostridium(7.25%)were the dominant genera.Microbial diversity analysis at the genus level found that the five livestock farms shared the most microorganisms at the genus level,with 3357 genera in common.Microbial gene differential analysis revealed that the five livestock farms shared a relatively small number of common genes(1065).Moreover,the functional prediction results also showed that 29.10%of the annotated genes were related to metabolism,such as carbohydrate metabolism,amino acid metabolism,and lipid metabolism.The analysis of the differences in gene number showed that increased similarity in the species composition of Culicoides was associated with a greater number of genes shared by the samples.Conclusions Analysis of the microbial communities found that the core symbiotic microflora communities in Culicoides is relatively stable,and livestock farms with the same dominant species of Culicoides have more similarities in microbial communities in Culicoides.This study enriches the microbial community database of Culicoides in China,which is of great significance for the biological prevention and control of Culicoides in the future.