妊娠合并慢性乙型肝炎患者乙肝标志物、前S1抗原与HBV-DNA的相关性

Correlation between Hepatitis B markers,pre-S1 antigen and HBV-DNA in patients with pregnancy complicated with chronic Hepatitis B and its application value

目的探讨妊娠合并慢性乙型肝炎患者乙肝标志物与HBV-DNA载量的相关性。方法选择收治的120例妊娠合并慢性乙型肝炎患者,采用酶联免疫吸附法检测乙肝五项(HBV-M)和乙肝前S1抗原(Pre-S1),采用荧光定量聚合酶链反应法(PCR)检测HBV-DNA,对结果行相关性分析。结果HBsAg、HBeAg、HBcAb阳性模式下HBV-DNA阳性率为80.49%,Pre-S1阳性率为75.61%,组间差异无统计学意义(P>0.05);HBsAg、HBeAb、HBcAb阳性模式下HBV-DNA阳性率为42.50%,Pre-S1阳性率为35.00%,组间差异无统计学意义(P>0.05);Pre-S1阳性检出率在HBsAg、HBsAb、HBeAg、HBeAb、HBcAb阳性和阴性标本中差异均有统计学意义(P<0.05)。经Pearson相关性分析,HBeAg、HBsAg与Pre-S1呈正相关关系;HBeAb与Pre-S1呈负相关关系;HBV-DNA与Pre-S1为正相关关系。结论妊娠合并慢性乙肝患者HBV-M、Pre-S1与HBV-DNA相关性高,对慢性乙肝的预测具有一定意义。

Objective To investigate the correlation between hepatitis B markers and HBV-DNA load in patients with pregnancy complicated with chronic hepatitis B.Methods A total of 120 pregnant patients with chronic hepatitis B admitted to our department were enrolled in this study.Enzyme-linked immunosorbent assay(ELISA)was used to detect HBV-M and pre-S1 antigen(Pre-S1).The method(PCR)was used to detect HBV-DNA,and the results were correlated.Results The positive rate of HBV-DNA in HBsAg,HBeAg and HBcAb positive patterns was 80.49%,and the positive rate of Pre-S1 was 75.61%.The difference was not statistically significant(P>0.05).The positive rate of HBV-DNA in HBsAg,HBeAb and HBcAb positive models was 42.50%,and the Pre-S1 positive rate was 35.00%,and there was no significant difference between the two groups(P>0.05).The positive pre-S1 detection rate was significantly different in HBsAg,HBsAb,HBeAg,HBeAb,HBcAb positive and negative samples(P<0.05).According to Pearson correlation analysis,HBeAg,HBsAg and Pre-S1 were positively correlated;HBeAb and Pre-S1 were negatively correlated;and HBV-DNA was positively correlated with Pre-S1.Conclusion HBV-M and Pre-S1 are associated with HBV-DNA in patients with chronic hepatitis B and have a certain significance for the prediction of chronic hepatitis B.