3D打印制备镁黄长石生物陶瓷骨组织工程支架及其性能

Akermanite Scaffolds for Bone Tissue Engineering:3D Printing Using Polymer Precursor and Scaffold Properties

具备良好成骨性能和降解速率的生物陶瓷骨组织工程支架在骨修复领域极具应用潜力。镁黄长石(Ca_(2)MgSi_(2)O_(7))因其具有良好的力学性能、生物降解能力以及促成骨性能而备受关注。本研究以硅树脂为聚合物前驱体、碳酸钙与氧化镁为活性填料制备打印浆料,采用挤出式3D打印技术在室温条件下制备支架素坯,并在惰性气氛下高温烧结制备了镁黄长石生物陶瓷支架,并对比研究了镁黄长石支架与斜硅钙石(Ca_(2)SiO_(4))、镁橄榄石(Mg_(2)SiO_(4))支架在结构、抗压强度、体外降解能力以及体外生物学性能等方面的差异。结果表明:镁黄长石支架与斜硅钙石、镁橄榄石支架具有相似的三维多孔结构,抗压强度、降解速率介于镁橄榄石和斜硅钙石之间,但促进骨髓间充质干细胞的成骨基因表达能力显著强于镁橄榄石和斜硅钙石支架。本研究证实采用3D打印制备的镁黄长石支架有望作为骨组织工程较理想的支架。

Bioceramic scaffolds with excellent osteogenesis ability and degradation rate exhibit great potential in bone tissue engineering.Akermanite(Ca_(2)MgSi_(2)O_(7))has attracted much attention due to its good mechanical property,biodegradability and enhanced bone repair ability.Here,akermanite(Ca_(2)MgSi_(2)O_(7))scaffolds were fabricated by an extrusion-type 3D printing at room temperature and sintering under an inert atmosphere using printing slurry composed of a silicon resin as polymer precursor,and CaCO_(3)and MgO as active fillers.Furthermore,the differences in structure,compressive strength,in vitro degradation,and biological properties among akermanite,larnite(Ca_(2)SiO_(4))and forsterite(Mg_(2)SiO_(4))scaffolds were investigated.The results showed that the akermanite scaffold is similar to those of larnite and forsterite in 3D porous structure,and its compressive strength and degradation rate were between those of the larnite and forsterite scaffolds,but it showed a greater ability to stimulate osteogenic gene expression of rabbit bone marrow mesenchymal stem cells(rBMSCs)than both larnite and forsterite scaffolds.Hence,such 3D printed akermanite scaffold possesses great potential for bone tissue engineering.