双相磷酸镁钙微球体外成血管和促成骨研究

In vitro Study of Biphasic Calcium Magnesium Phosphate Microspheres for Angiogenesis and Bone Formation

磷酸三钙(β-TCP)陶瓷替代材料由于其与骨矿物成分相近及良好的生物相容性和骨传导性,近年来被广泛关注,常以纳米颗粒、支架和微球等形式用于骨修复。本研究制备了五种不同的磷酸三钙/磷酸三镁(TMP)(TCP、25%TMP、50%TMP、75%TMP和TMP)复合微球并作了相应表征。随着复合微球中TMP含量增加,微球释放的Mg^(2+)和Ca^(2+)的累积浓度增加,且TMP可以调节复合微球的降解速率。以小鼠胚胎成骨细胞前体细胞(MC3T3-E1)和人脐静脉内皮细胞(HUVECs)为模型,评价了该复合微球的生物相容性、成血管和成骨作用。结果表明,与TCP、TMP和75%TMP相比,25%TMP和50%TMP复合微球具有更好的细胞相容性,对HUVECs有一定的促增殖作用。因此,含25%TMP和50%TMP的复合微球对血管生成和成骨具有更积极的作用。

Beta tricalcium phosphate(β-TCP)ceramic substituted materials have attracted a large amount of atten-tion in the last decades because of their chemical similarity with bone inorganic components,good biocompatibility,and osteoconductivity.Such materials can be used for bone replacement and bone formation in various forms,such as nanoparticles,scaffolds and microspheres.In this study,five different microsphere materials of tricalcium phosphate/trimagnesium phosphate(TMP)(TCP,25%TMP,50%TMP,75%TMP,and TMP)composites were prepared and characterized.With the increase of TMP content in the composite microspheres,the cumulative concentration of Mg^(2+)and Ca^(2+)released from the microspheres increased,indicating that TMP can regulate the degradation rate of the composite microspheres.The osteoblast precursor cell line(MC3T3-E1 cells)and human umbilical vein endothelial cells(HUVECs)were used as models to evaluate the biocompatibility,angiogenesis and osteogenesis of the composite microspheres.The results showed that compared with TCP,TMP and 75%TMP group,25%TMP and 50%TMP composite microspheres had better cell compatibility and had a certain proliferative effect on HUVECs.Therefore,composite microspheres of 25%TMP and 50%TMP have more significant positive effects on angiogenesis and osteogenesis.