鼻咽癌中miR-18a和缺氧诱导因子-1α的表达及其与放疗敏感性和预后的相关性

Expression of miR-18a and Hypoxia-inducible Factor-1αin Nasopharyngeal Carcinoma and Their Correlation with Radiosensitivity and Prognosis

目的探究鼻咽癌中miR-18a和缺氧诱导因子-1α的表达及其与放疗敏感性和预后的相关性。方法选取鼻咽癌患者91例。根据放疗敏感性的不同将患者分为放疗敏感组(n=24)和放疗抵抗组(n=67)。结果miR-18表达水平与鼻咽癌患者年龄、性别、T分期、M分期、N分期以及临床分期等临床参数无关(P>0.05);HIF-1α表达水平与鼻咽癌患者年龄、性别、T分期、M分期以及N分期等临床参数无关(P>0.05),但是与临床分期有关(P<0.05)。统计学分析两组年龄、性别、T分期、M分期、N分期以及临床分期的差异无统计学意义(P>0.05),化疗敏感组miR-18a、HIF-1α的表达水平明显低于化疗抵抗组,差异有统计学意义(P<0.05)。多因素分析结果发现,miR-18a、HIF-1α表达水平与放疗敏感性有关(P<0.05)。统计学分析发现复发、远处转移以及死亡患者的miR-18a、HIF-1α表达水平明显高于无复发、远处转移以及生存患者(P<0.05)。结论鼻咽癌患者miR-18a和缺氧诱导因子-1α水平与放疗敏感性有关,为今后探索鼻咽癌的预后指标提供了一定的参考依据。

Objective To investigate the expression of miR-18a and hypoxia-inducible factor-1αin nasopharyngeal carcinoma and their correlation with radiotherapy sensitivity and prognosis.Methods A total of 91 patients with nasopharyngeal carcinoma were selected.Patients were divided into radiotherapy sensitive group(n=24)and radiotherapy resistant group(n=67)according to different radiotherapy sensitivity.Results The expression level of miR-18 was not correlated with the age,gender,T stage,M stage,N stage and clinical stage of nasopharyngeal carcinoma patients(P>0.05).HIF-1αexpression level was not correlated with age,sex,T stage,M stage and N stage(P>0.05),but was correlated with clinical stage(P<0.05).There were no statistically significant differences in age,gender,T stage,M stage,N stage and clinical stage between the two groups(P>0.05).The expression levels of miR-18a and HIF-1αin the chemotherapy sensitive group were significantly lower than those in the chemotherapy resistant group,and the difference was statistically significant(P<0.05).The results of multivariate analysis showed that the expression levels of miR-18a and HIF-1αwere related to radiotherapy sensitivity(P<0.05).Statistical analysis showed that the expression levels of miR-18a and HIF-1αin patients with recurrence,distant metastasis and death were significantly higher than those in patients without recurrence,distant metastasis and survival(P<0.05).Conclusion The levels of miR-18a and hypoxia-inducible factor-1αin nasopharyngeal carcinoma patients are related to radiotherapy sensitivity,which provides a certain reference for future exploration of prognostic indicators of nasopharyngeal carcinoma.