Ca^(2+)对盐胁迫下金盏菊幼苗生理效应的影响

Ca^(2+)on the Physiological Effect of Calendula officinalis Seedling Under Salt Stress

以金盏菊幼苗为试材,采用盆栽方法,研究不同浓度(5、10、15、20 mmol·L^(-1))CaCl_(2)对100 mmol·L^(-1)NaCl胁迫下金盏菊幼苗叶绿素含量、丙二醛(MDA)含量、过氧化物酶(POD)活性、脯氨酸(Pro)含量变化和叶片膜透性等生理指标的影响,以期探究钙(Ca)对金盏菊幼苗耐盐性的缓解效应。结果表明:用不同浓度CaCl_(2)处理金盏菊,其中对幼苗生长缓解效果最好的是施加10 mmol·L^(-1)CaCl_(2)溶液的T2组,叶绿素含量增加了13.43%,MDA含量下降了34.72%,POD活性增加了42.69%,Pro含量降低了28.37%,相对电导率降低了30.20%。综上所述,钙可明显缓解盐胁迫给金盏菊幼苗生长带来的不良影响,以10 mmol·L^(-1)CaCl_(2)溶液处理效果最好。

The Calendula officinalis seedlings were used as experimental materials with the potting experiment.The effects of calcium chloride(CaCl_(2))with different concentrations(5,10,15,20 mmol·L^(-1))on the changes of chlorophyll content,malondialdehyde(MDA)content,peroxidase(POD)activity,proline(Pro)content and leaf membrane permeability in pot seedlings of Calendula officinalis seedlings under 100 mmol·L^(-1)sodium chloride(NaCl)stress were studied,in order to explore the mitigation effects of calcium on the salt tolerance of Calendula officinalis seedlings.The results showed that the treatment T2 with 10 mmol·L^(-1)CaCl_(2)solution had the best alleviating effect on Calendula officinalis seedlings growth.The chlorophyll content increased by 13.43%,MDA content decreased by 34.72%,and POD activity increased by 42.69%.Pro content and relative conductivity decreased by 28.37%and 30.20%respectively.In conclusion,calcium can obviously alleviate the adverse effects of salt stress on the growth ofCalendula officinalis seedlings,and 10 mmol·L^(-1)of CaCl_(2)solution had the best effect.