摘要
小反刍兽疫(peste des petits ruminants,PPR)是一种由小反刍兽疫病毒(peste des petits ruminants virus,PPRV)引起的急性、高度传染性疾病,主要感染山羊和绵羊,发病率和死亡率极高。由于其严重的病理损害及广泛的传播性,给各国养殖业及国际贸易造成巨大经济损失,因此,建立快速、准确的检测方法对该病的防控尤为重要。本文就用于PPRV检测的普通RT-PCR、普通多重PCR、实时荧光定量PCR(real-time fluorence quantitative PCR,RT-qPCR)、焦磷酸光化测序、巢式PCR(nested PCR,nPCR)、环介导等温扩增(loop-mediated isothermal amplification,LAMP)、重组酶聚合酶扩增(recombinase polymerase amplification,RPA)等生物检测技术的研究进展作一综述,以期为科学检测、鉴定PPRV提供依据和思路。
Peste des petits ruminants(PPR)is an acute and highly contagious disease caused by peste des petits ruminants virus(PPRV),which mainly infects goats and sheep with high morbidity and mortality. Because of its serious pathological damage and wide spread,PPR has caused huge economic losses to the aquaculture industry and international trade in various countries,so it is particularly important to establish a rapid and accurate detection method for the prevention and control of the disease. This paper reviews the progress in research on biological detection techniques for PPRV detection,such as routine RT-PCR,routine multiplex PCR,real-time fluorence quantitative PCR(RT-qPCR),pyrosequencing,nested PCR(nPCR),loop-mediated isothermal amplification(LAMP)and recombinase polymerase amplification(RPA),so as to provide bases and ideas for scientific detection and identification of PPRV.
作者
何云江
贾伟娟(综述)
郗珊珊
王学理(审校)
HE Yunjiang;JIA Weijuan;CHI Shanshan;WANG Xueli(College of Animal Science and Technology,Inner Mongolia Minzu University,Tongliao 028000,Inner Mongolia Autonomous Region,China)
出处
《中国生物制品学杂志》
CAS
CSCD
北大核心
2023年第8期1014-1020,共7页
Chinese Journal of Biologicals
基金
内蒙古自治区高等学校科学技术研究重点项目(NJZZ19147)
内蒙古自治区肉牛疾病防控工程技术研究中心开放课题(MDK-2019022)。
关键词
小反刍兽疫病毒
检测技术
分子生物学
Peste des petits ruminants virus(PPRV)
Detection techniques
Molecular biology