阵列式碳点印迹纸芯片快速检测槐花中芦丁含量

Rapid detection of rutin in sophora japonica by array carbon dot molecularly imprinted paper chip

基于芦丁对碳点的荧光猝灭作用,结合分子印迹技术制备了芦丁选择性检测纸芯片。以壳聚糖为功能单体,芦丁为模板分子,天冬酰胺热裂解获得的碳点为核,戊二醛为交联剂,进行分子印迹,将该印迹预聚液滴于经激光打印处理过的滤纸阵列式亲水区,制得低成本的碳点印迹纸芯片。通过分析显色斑点三原色R(红)、G(绿)、B(蓝)值,对纸芯片的制备条件和显色条件进行了优化。结果表明在优化条件下,含结构类似物在内的共存物质不干扰测定,荧光斑点的R+G+B值与芦丁浓度在0.01~0.12 mg·mL^(-1)区间时呈良好的线性关系,R^(2)=0.991。按此法测得槐花中芦丁含量的平均相对标准偏差为6.8%,回收率为87.75%~105.7%,与HPLC测定结果接近。该纸芯片能用于复杂样品中芦丁含量的快速现场测定。

Based on the fluorescence quenching effect of rutin on carbon dots,combined with molecular imprinting technology,a paper chip for selective detection of rutin was prepared.The carbon powder was printed on the filter paper by a laser printer to obtain a paper chip with array hydrophilic and hydrophobic regions.Using chitosan as the functional monomer,rutin as the template molecule,carbon dots obtained by thermal cracking as the core,and glutaraldehyde as the cross-linking agent,molecular imprinting was carried out,and the imprinted prepolymer was dropped on array hydrophilic regions of the filter paper to obtain carbon dot imprinted paper chips.By analyzing the three primary colors R(red),G(green)and B(blue)values of the color spots,the preparation conditions and color development conditions of the paper chips were optimized.The results showed that under the optimized conditions,the coexisting substances did not interfere with the determination,and the R+G+B value of the fluorescent spots had a good linear relationship with the rutin concentration in the range of 0.01~0.12 mg·mL^(-1),with R^(2)=0.991.According to this method,the average relative standard deviation of rutin content in Sophora japonica was 6.8%,and the recovery rate was 87.75%~105.7%,which was close to the HPLC determination result.The paper chip can be used for rapid on-site determination of rutin content in complex samples.