长非编码RNA MIR210HG在宫颈癌组织中的表达及相关竞争性内源RNA机制

Comprehensive study on the expression and relative competing endogenous RNA mechanisms of long non-coding RNA MIR210HG in cervical cancer

目的整合计算多中心样本探究长非编码RNA MIR210HG在宫颈癌组织中的表达及竞争性内源RNA机制。方法收集来自TCGA、GEO、SRA、ArrayExpress等数据库中与宫颈癌相关的lncRNA(long noncoding RNA,长非编码RNA)高通量数据集,利用t检验比较各数据集中MIR210HG在宫颈癌组织与非癌对照中表达的差异。整合计算标准化均数差(standard mean difference,SMD)以综合评估MIR210HG在宫颈癌组织中的表达。利用在线工具预测MIR210HG的靶miRNA以及相关靶基因。结果整合计算407例样本的SMD,结果提示MIR210HG在宫颈癌组织中的表达水平显著上调(SMD=0.57,95%CI:0.33~0.81,P<0.05)。通过结合位点预测以及整合计算SMD,MIR210HG的靶miRNA hsa-miR-424-5p在宫颈癌组织中低表达,其靶基因MYB在宫颈癌组织中高表达。结论MIR210HG在宫颈癌组织中的表达显著上调,其可能通过MIR210HG/hsa-miR-424-5p/MYB轴参与宫颈癌的发生与发展。

Objective To investigate the expression and relative competing endogenous RNA(ceRNA)mechanisms of long non-coding RNA(lncRNA)MIR210HG by integrated calculation.Methods LncRNA-related high-throughput datasets of cervical cancer were collected from TCGA,GEO,SRA and ArrayExpress database.Student′s t-test was used to compare the expression level of MIR210HG between cervical cancer and non-tumor cervix in each dataset.Meanwhile,integrated standard mean difference(SMD)was calculated to assess the expression of MIR210HG in cervical cancer comprehensively.Online tools DIANA-LncBase and TargetScan Human was used to predict the target miRNAs of MIR210HG and target genes of miRNA.Results The pooled SMD(SMD=0.57,95%CI:0.33-0.81,P<0.05)indicated that MIR210HG was upregulated in cervical cancer tissues via integrated calculation with 407 cases(316 cases of cervical cancer and 91 cases of normal cervix).Three putative target miRNAs were included via prediction of binding sites and integrated calculation of SMD(hsa-miR-424-5p,hsa-miR-218-5p and hsa-miR-3194-5p).Simutaneously,pooled calculation verified that hsa-miR-424-5p was downregulated while its target gene MYB was overexpressed in cervical cancer tissues.Conclusions MIR210HG was significantly upregulated in cervical cancer tissues and it may participate in the development of cervical cancer viva MIR210HG/hsa-miR-424-5p/MYB axis,which is worthy of further validation.