血型基因检测辅助高频抗原抗体的鉴定——附一例报道

Identification of antibody against highly prevalent antigen through serological test and molecular biology technology

目的利用血型基因检测技术辅助鉴定1例高频抗原抗体。方法采用血清学试验进行血型鉴定、特异性抗体鉴定及交叉配血,采用分子生物学技术进行Diego血型基因检测。结果血清学试验结果提示患者血清中存在高频抗原抗体抗-Dib;基因测序结果显示患者Diego基因型为Di(a+b-);从856名献血者中筛选到2名献血者血液与患者相配合,基因型均为Di(a+b-)。结论以血清学试验为主、分子生物学技术为辅的联合检测手段,有助于高频抗原抗体的检出,对保障临床输血安全具有至关重要的意义。

Objective To identify a case of antibody against highly prevalent antigen through molecular biology technology.Methods Blood group typing,unexpected antibody identification and cross matching were performed by serological test,and genetic testing of Diego blood group was performed by molecular biology technology.Results Serological test showed that there was a high prevalence of anti-Dib in the serum of the patient.Gene sequencing showed that the genotype of the patient was Di(a+b-).Two cases with Di(a+b-)matched with the patient were screened from 856 blood donors.Conclusion The combined detection method based on serological test supplemented by molecular biology technology is beneficial to the detection of antibody against highly prevalent antigens,and is of great significance for ensuring the safety of clinical blood transfusion.