基于转录组测序的粗肋草SSR位点分析和分子标记开发

SSR Loci Analysis and Molecular Marker Development in Aglaonema commutatum Based on Transcriptome Sequencing

粗肋草是常见的室内观赏植物,为了规范其商业品种的开发,以及为后续的分子育种研究提供基础数据,本研究开发了高效可靠、操作简单SSR分子标记。从粗肋草‘如意’转录组测序获得到的115 449条unigene中共检测到29 776个SSR位点,优势重复类型为单核苷酸重复和二核苷酸重复,分别占总SSR位点的42.30%和40.05%。以8个品种粗肋草DNA为模板,设计的55对SSR引物中有14条的扩增产物经3%琼脂糖凝胶电泳后呈现出清晰单一的条带;经毛细管凝胶电泳后获得了12对具有多态性的引物。基于12对引物扩增片段的35个等位位点,通过UPGMA聚类分析将8个品种粗肋草分为3个类群,其中,‘红如意’和‘吉利红’可能为同种异名。本研究筛选出的SSR引物可为粗肋草的遗传多样性分析、遗传图谱构建、遗传稳定性鉴定等提供可靠的分子标记。

Aglaonema commutatum is a common indoor ornamental plant.In order to regulate the development of commercial varieties and provide basic data for subsequent molecular breeding research,this study developed an efficient,reliable and simple SSR molecular marker.29 776 SSRs were discovered in 115 449 unigenes of transcriptome of Aglaonema commutatum 'Red valentine',and the major repeat motif types were mononucleotide and dinucleotide,accounting for 42.30% and 40.05% respectively.14 pairs of primers whose PCR products showed clear and single fragment in AGE were selected from 55 SSR primers while using DNA of 8 varieties of Aglaonema commutatum as templates.Furthermore,12 pairs of primers showed polymorphic fragments in HPCE.Based on 35 alleles from fragments of 12 pairs of primers,8 varieties of Aglaonema commutatum were divided into3 groups through UPGMA clustering analysis,in which 'Redder valentine' and 'Big apple' may be the same variety.SSR primers selected from this study could provide reliable and effective markers for analysis of genetic polymorphism,genetic stability test in Aglaonema commutatum.