IL-9、IL-17及TGF-β1在复发转移肝泡状棘球蚴病中的表达及临床意义

Expression and clinical significance of IL-9,IL-17 and TGF-β1 in recurrent and metastatic hepatic alveolar echinococcosis

目的观察白细胞介素-9(IL-9)、白细胞介素-17(IL-17)及转化生长因子β1(TGF-β1)在人体肝泡状棘球蚴病复发转移中的表达及意义。方法选取61例未复发转移和32例复发转移的人感染泡状棘球蚴病例,采用免疫组化(Immunohistochemistry,IHC)方法检测初次肝泡状棘球蚴病灶旁炎症细胞中IL-9、IL-17和TGF-β1的蛋白表达,运用Aipathwell分析其表达程度及与临床术后复发转移的关系。结果IL-9和IL-17在肝泡状棘球蚴病术后复发转移组织中的表达水平高于未复发转移组织,差异有统计学意义(P<0.05),IL-9与IL-17的表达呈正相关(r=0.377,P<0.001);TGF-β1蛋白表达水平在复发转移组织与未复发转移组织间无明显差异(P>0.05)。单因素分析显示IL-9、职业、年龄、婚姻状况与肝泡状棘球蚴病术后复发转移相关。多因素Cox回归分析显示职业是肝泡状棘球蚴病患者术后复发转移的危险因素。结论IL-9、IL-17在肝泡状棘球蚴病复发转移组织中呈高表达,并呈正相关,提示两者可能在参与肝泡状棘球蚴病患者复发转移的过程中产生作用。IL-9可作为肝泡状棘球蚴病复发转移的参考指标。从事农牧民工作是其复发转移的危险因素。

Objective To investigate the expression and significance of interleukin-9(IL-9),interleukin-17(IL-17)and transforming growth factorβ1(TGF-β1)in the recurrence and metastasis of human hepatic alveolar echinococcosis.MethodsThe protein expressions of IL-9,IL-17 and TGF-β1 in inflammatory cells adjacent to the primary hepatic alveolar echinococcus lesions were detected by immunohistochemistry(IHC)in 61 cases of human infection with alveolar echinococcus without recurrence and metastasis and 32 cases of human infection with recurrence and metastasis.The degree of expression and the relationship with clinical postoperative recurrence and metastasis was analyzed by Aipathwell.ResultsThe expression levels of IL-9 and IL-17 in the recurrent and metastatic tissues of hepatic alveolar Echinococcosis after the operation were higher than those in the non-recurrent and metastatic tissues(P<0.05),and IL-9 and IL-17 were positively correlated(r=0.377,P<0.001);the expression levels of TGF-β1 protein were not significantly different between the recurrent and non-recurrent metastatic tissues(P>0.05).Univariate analysis log-rank test showed that IL-9,occupation,age and marital status were associated with postoperative recurrence and metastasis of hepatic alveolar echinococcosis.Multivariate Cox regression analysis showed that occupation was a risk factor affecting recurrence and metastasis in patients with hepatic alveolar echinococcosis.ConclusionIL-9 and IL-17 were highly expressed in the recurrence and metastasis of hepatic alveolar echinococcosis,and there was a positive correlation,suggesting that IL-9 and IL-17 may play a synergistic role in the recurrence and metastasis of hepatic alveolar echinococcosis.IL-9 can be used as a reference index for the recurrence and metastasis of hepatic alveolar echinococcosis.Working as farmers and herdsmen is a risk factor for its recurrence and metastasis.