响应面优化橄榄仁红皮黄酮提取及组成分析

Optimization of extraction and composition analysis of flavonoids from olive kernels by response surface

以橄榄仁红皮为原材料,采用乙醇-水溶液提取活性较强的天然黄酮类化合物,通过单因素提取实验和响应面实验,得出最佳提取条件,以抗坏血酸为对照,对提取物进行抗氧化活性测定,并采用Thermo超高效液相色谱系统对提取的活性物质进行鉴定。结果表明,橄榄仁红皮黄酮类化合物(flavonoid from red skin of olive kernel,FRSOK)最佳的提取条件为浸提温度57.61℃,乙醇体积分数为75%,浸提时间为35 min,预测总黄酮含量243.53μg/mL。天然的FRSOK清除羟自由基能力较强,清除DPPH自由基能力次之,清除超氧阴离子自由基能力最弱。通过分析以及对比,确定了橄榄仁红皮含有21种黄酮类化合物,包括新橙皮苷、槲皮素、山奈酚、异鼠李素等以及各自的衍生物。因此,FRSOK可作为抗氧化产品或其替代品应用到食品工业中,具有良好的应用前景。

Taking olive kernel red peel as raw material,ethanol-water solution was used to extract natural flavonoids with strong activity,and the optimal extraction conditions were obtained through one-factor extraction experiments and response surface experiments,and the antioxidant activity of the extract was determined by ascorbic acid as a control,and the extracted active substances were identified by thermo ultra-high performance liquid chromatography system.Results showed that the optimal extraction conditions for olive kernel red flavonoids(FRSOK)were an extraction temperature of 57.61℃,an ethanol volume fraction of 75%,an extraction time of 35 min,and a predicted total flavonoid content of 243.53μg/mL.Natural FRSOK in·OH scavenging ability was strong,followed by DPPH free radical scavenging ability,and·O~-_(2)scavenging ability was the weakest.Through analysis and comparison,it was determined that the red peel of olive kernel contained 21 kinds of flavonoids,including neohesperidin,quercetin,kaempferol,isorhamnetin,etc.,and their respective derivatives.Therefore,FRSOK can be applied to the food industry as an antioxidant product or its substitute,with good application prospects.