Kruppel样因子4和骨桥蛋白在结直肠癌组织的表达及其临床意义

Expression of Krippel-like factor 4 and osteopontin in colorectal cancer and their relationship with clinicopathological features

目的探讨Krüppel样因子4(KLF4)和骨桥蛋白(OPN)在结直肠癌组织的表达水平及其与结直肠癌患者临床病理特征和预后的关系。方法收集2017年1月至2022年12月丽水市人民医院收治的130例结直肠癌组织和对应癌旁组织标本, 采用免疫组织化学法检测KLF4蛋白和OPN蛋白在上述组织的表达, 应用χ^(2)检验比较KLF4蛋白、OPN蛋白的表达及其与结直肠癌临床病理特征的关系。结果 KLF4蛋白在结直肠癌组织中表达率为32.31%(42/130), 明显低于对应癌旁组织[77.69%(101/130)], 两者差异有统计学意义(χ^(2)=54.095, P<0.01)。KLF4表达水平与结直肠癌患者浸润深度、淋巴结转移、TNM分期、组织学分化程度明显相关(χ^(2)=4.796、5.461、7.718、5.128, P<0.05), KLF4表达水平与结直肠癌患者性别、年龄、肿瘤部位无相关(χ^(2)=0.001、0.018、0.030, P>0.05)。OPN蛋白在结直肠癌组织中表达率为47.69%(62/130), 明显高于对应癌旁组织[16.15%(21/130)], 两者比较差异有统计学意义(χ^(2)=29.750, P<0.01)。OPN表达水平与结直肠癌患者TNM分期、淋巴结转移、浸润深度明显相关(χ^(2)=4.182、5.058、5.112, P<0.05), OPN表达水平与结直肠癌患者性别、肿瘤部位、组织学分化程度、年龄无相关(χ^(2)=0.002、0.112、0.395、0.034, P>0.05)。结论结直肠癌组织中KLF4表达下调、OPN表达上调, 检测KLF4和OPN的表达水平有助于预测结直肠癌患者的预后。

Objective To investigate the expression levels of Kruppel-like factor 4(KLF4)and osteopontin(OPN)in colorectal cancer tissues and their relationship with clinicopathological characteristics and prognosis of patients with colorectal cancer.Methods Totally,130 cases of colorectal cancer tissues and adjacent normal colorectal tissues in Lishui People's Hospital from January 2017 to December 2022 were collected.Immunohistochemistry was used to detect the expression of KLF4 protein and OPN protein in the above tissues.Chi-square test was used to compare the expression of KLF4 protein and OPN protein and their relationship with clinicopathological characteristics of colorectal cancer.Results The expression rate of KLF4 protein in colorectal cancer tissues was 32.31%(42/130),which was significantly lower than that in adjacent normal colorectal tissues[77.69%(101/130),χ^(2)=54.095,P<0.01].The expression level of KLF4 was significantly correlated with the depth of invasion,lymph node metastasis,TNM stage and histological differentiation of colorectal cancer patients(respectively,χ^(2)=4.796,5.461,7.7185.128,P<0.05),but not significantly with gender,age and tumor location of colorectal cancer patients(respectively,χ^(2)=0.001,0.018,0.030,P>0.05),The expression rate of OPN protein in colorectal cancer tissues was 47.69%(62/130),which was significantly higher than that in adjacent normal colorectal tissues[16.15%(21/130),χ^(2)=29.750,P<0.01].The expression level of 0PN was significantly correlated with TNM stage,lymph node metastasis and depth of invasion in patients with colorectal cancer(respectively,χ^(2)=4.182,5.058,5.112,P<0.05),but not significantly with gender,tumor location,histological differentiation and age of colorectal cancer patients(respectively,χ^(2)=0.002,0.112,0.395,0.034,P>0.05).Conclusion KLF4 expression is down-regulated and OPN expression is up-regulated in colorectal cancer tissues.Detection of KLF4 and OPN expression levels may have certain clinical signifi-cance in predicting the prognosis of patients with colorectal cancer.