虫草素抑制TNF⁃α诱导的人角质形成细胞系HaCaT增殖

Cordycepin inhibits proliferation of human keratinocyte cell line HaCaT induced by TNF-α

目的探讨虫草素对肿瘤坏死因子α(TNF-α)诱导人角质形成细胞系HaCaT增殖、凋亡和炎性反应的影响。方法将HaCaT细胞分为对照组(control)、模型组(model)及低、中、高浓度虫草素干预组(25μmol/L、50μmol/L、100μmol/L),先用40μg/L TNF-α对模型组、虫草素干预组诱导24 h,制作银屑病(PS)样细胞模型;之后虫草素干预组加入相应浓度的虫草素,继续培养24 h;用CCK-8法检测细胞增殖,用流式细胞仪检测细胞凋亡,用RT-qPCR检测炎性细胞因子白介素(IL)-8、IL-1βmRNA相对表达量。结果虫草素干预24 h后细胞增殖率下降(P<0.05),细胞凋亡率升高(P<0.05),IL-8和IL-1β的mRNA表达水平下降(P<0.05)。结论虫草素可抑制TNF-α诱导的人角质形成细胞系增殖、炎性反应并促进细胞凋亡。

Objective To study the effect of cordycepin on proliferation,apoptosis and inflammatory reaction of human keratinocyte cell line HaCaT induced by TNF-α.Methods HaCaT cells were divided into control group,model group and intervention groups including low,middle,high dose(cordycepin 25μmol/L,50μmol/L and 100μmol/L,respectively).HaCaT cells were incubated with by 40μg/L TNF-αin model group and cordycepin intervention group for 24 hours.Then,the cordycepin intervention groups were incubated with corresponding cordycepin for 24 hours.Cell proliferation was detected by CCK-8 method.Apoptosis was detected by flow cytometry.And mRNA expression of interleukin(IL)-8,IL-1βwas detected by RT-qPCR.Results After 24 hours of cordycepin incubation,cell proliferation rate decreased(P<0.05),cell apoptosis rate increased(P<0.05)and mRNA expression of IL-8 and IL-1βdecreased(P<0.05).Conclusions Cordycepin can inhibit TNF-α-induced proliferation and inflammatory reaction of HaCaT cells and promote apoptosis of these cells.