盐胁迫下不同花生品种的类黄酮含量和抗氧化酶活性

Flavonoid contents and antioxidant enzyme activities of different peanut cultivars under salt stress

花青素等黄酮类物质,既是人体所需的营养保健成分,也是介导植物适应逆境胁迫的重要代谢物。以3个不同种皮颜色的花生品种为研究对象,在苗期以150 mmol/L的NaCl进行盐胁迫处理,测定植株性状、类黄酮含量和抗氧化酶活性,分析不同品种的抗氧化能力和耐盐性。结果表明,盐胁迫抑制了株高、叶面积和生物量,3个品种的耐盐系数从高到低依次为济花黑1号、济花红1号、远杂9102。与远杂9102相比,济花红1号和济花黑1号MDA含量相对较低。盐胁迫显著提高了济花红1号和济花黑1号根系的SOD、POD、CAT等抗氧化酶活性。济花红1号和济花黑1号根系类黄酮含量和在盐胁迫下的相对值大于远杂9102。相关分析表明,相对类黄酮含量和MDA含量与耐盐系数显著相关,类黄酮与SOD活性、MDA含量、株高和叶面积相对值极显著相关。盐胁迫引起济花红1号和济花黑1号类黄酮大量积累,激活了抗氧化保护能力,可有效降低MDA的积累,减轻氧化损伤,缓解盐胁迫对植株生长的抑制作用。研究结果为筛选和推广耐盐碱的彩色花生提供了依据。

As nutritions for human health,anthocyanins and other flavonoids are important molecules that me-diate plant adaptation to environmental stress.The objective of this study was to investigate oxidation resistance and salt tolerance of 3 peanut cultivars with pink(YZ9102),red(JHR1),and black(JHB1)testa colors.A pot experiment was conducted under controlled conditions.7-day old seedlings were subjected to Hoagland solution(CK)and 150 mmol/L NaCl(SS)for 10 days.The changes in growth,flavonoid content,and antioxidant enzyme activities were ana-lyzed.Results showed that salt stress caused a decrease in plant height,leaves area and biomass in the 3 cultivars.JHR1 and JHB1 exhibited higher salt tolerance than YZ9102.The antioxidant enzyme activities of superoxide dis-mutase(SOD),peroxidase(POD)and catalase(CAT)of JHR1 and JHB1 were markedly increased by salt stress com-pared to YZ9102.The flavonoid contents exhibited the same trends in the 3 cultivars under salt stress.The relative malonaldehyde(MDA)content was lower in JHR1 and JHB1 than in YZ9102.Correlation analysis showed that the relative flavonoid content and MDA content were significantly(P<0.05)related to salt tolerance.The flavonoid con-tent was highly correlated(P<0.01)with the SOD activity,MDA content,plant height and leaf area.In conclusion,the roots of JHR1 and JHB1 accumulated more flavonoids than YZ9102 under salt stress and showed higher antioxi-dant capacity and less inhibition of growth under salt stress.The results might provide information for screening salt resistant peanut cultivars.