纳米溃疡液治疗大鼠放射性食管炎药效学和机制研究

Study on pharmacodynamics and mechanism of nano-Kuiyangye in treating radiation esophagitis in rats

目的:探究纳米溃疡液治疗Wistar大鼠放射性食管炎疗效,探索其可能机制。方法:使用30 Gy射线局部照射Wistar大鼠,构建大鼠放射性食管炎动物模型。照射后应用纳米溃疡液、中药溃疡液、康复新液、纳米水滑石基质、蒸馏水连续干预7 d,期间记录大鼠体重、进食量。第7天麻醉下腹主动脉取血,离心获得血清,ELISA法检测疼痛相关因子前列腺素-2(PGE-2)、P物质(SP)、降钙素基因相关肽(CGRP)表达量。取血后处死大鼠取全长食管组织,行苏木精-伊红(HE)染色分析大鼠食管的病理变化,Western Blotting法检测核因子-κB(NF-κB)炎症蛋白表达。结果:造模后大鼠造模后7天内总进食量、体重较对照组明显下降(P<0.05),疼痛相关因子PGE-2、SP、CGRP表达量较对照组升高(P<0.05),食管病理损伤评分升高(P<0.05),NF-κB炎症蛋白表达升高(P<0.05);治疗后,纳米溃疡液干预组大鼠造模后7天内总进食量高于其他各组(P<0.05),疼痛相关因子PGE-2、SP、CGRP表达量较模型组降低(P<0.05),食管病理损伤评分降低(P<0.05),NF-κB炎症蛋白表达降低(P<0.05)。结论:纳米溃疡液提高放射性食管炎大鼠进食量,减轻大鼠食管组织损伤,降低血清疼痛因子浓度;纳米溃疡液抗炎可能与降低NF-κB炎症因子水平相关。

Objective:To explore the curative effect of nano-Kuiyangye on radiation esophagitis in Wistar rats,and to explore its possible mechanism.Methods:Wistar rats were irradiated locally with 30Gy rays to establish an animal model of radiation esophagitis in rats.After irradiation,nano-Kuiyangye,traditional Chinese medicine ulcer solution,Kangfuxin solution,nano-hydrotalcite matrix,and distilled water were used to intervene continuously for 7 days,during which the body weight and food intake of the rats were recorded.On day 7,blood was collected from the abdominal aorta under anesthesia,and the serum was obtained by centrifugation.The expression levels of pain-related factors prostaglandin-2(PGE-2),substance P(SP),and calcitonin gene-related peptide(CGRP)were detected by ELISA.Rats were sacrificed after blood collection,and full-length esopha-geal tissues were taken.Hematoxylin-eosin(HE)staining was performed to analyze the pathological changes of the rat esophagus,and Western Blotting was used to detect the expression of nuclear factor-κB(NF-κB)inflammatory protein.Results:Compared with the control group,the total food intake and body weight of the rats within 7 days after modeling were significantly decreased(P<0.05),and the expressions of pain-related factors PGE-2,SP,and CGRP were higher than those of the control group(P<0.05).,the esophageal pathological damage score increased(P<0.05),and the expression of NF-κB inflammatory protein in-creased(P<0.05);after treatment,the total food intake of the rats in the nano-Kuiyangye intervention group within 7 days after modeling was higher than that in other groups(P<0.05),the expressions of pain-related factors PGE-2,SP,and CGRP were lower than those in the model group(P<0.05),the esophageal pathological damage score was lower(P<0.05),and the expres-sion of NF-κB inflammatory proteins was lower(P<0.05).Conclusion:nano-Kuiyangye increases the food intake of rats with ra-diation esophagitis,reduces esophageal tissue damage,and reduces the concentration of serum pain factors;the anti-inflammatory effect of nano-Kuiyangye may be related to the reduction of NF-κB inflammatory factor levels.