QuEChERS净化-气相色谱/三重四极杆质谱法测定酱油中6种对羟基苯甲酸酯类

Determination of 6 Parabens in Soy Sauce by QuEChERS Purification GC-MS/MS

试验建立了QuEChERS净化-气相色谱/三重四极杆质谱仪检测酱油中6种对羟基苯甲酸酯类含量的方法。样品经盐酸酸化、乙腈提取、QuEChERS净化,用气相色谱/三重四极杆质谱仪检测,外标法定量。本方法实验条件下,对羟基苯甲酸酯类在0.02~5.0 mg/L范围内呈良好线性关系,相关系数r2≥0.9991;样品中加标回收率为78.3%~101.3%,相对标准偏差介于1.5%~4.2%,对羟基苯甲酸酯类的检出限为0.01 mg/kg。该方法操作简便,重现性好,且准确度、灵敏度高。

A QuEChERS purification GC-MS/MS method was established for the determination of 6 parabens in soy sauce.The samples were acidified by hydrochloric acid,extracted by acetonitrile,purified by QuEChERS,detected by GC-MS/MS,and quantified by external standard method.Under the experimental conditions of this method,there was a good linear relationship between parabens in the range of 0.02~5.0 mg/L,and the correlation coefficient r2≥0.9991.The recoveries ranged from 78.3%to 101.3%,and the relative standard deviations ranged from 1.5%to 4.2%.The detection limits of parabens were 0.01 mg/kg.This method is simple in operation,good in reproducibility,high in accuracy and sensitivity.