清肺排毒汤高效液相色谱指纹图谱的建立及主成分分析研究

Establishment of High Performance Liquid Chromatography Fingerprint of Qingfei Paidu Decoction and Principal Component Analysis Study

为抗新型冠状病毒肺炎(COVID-19)方药清肺排毒汤的质量控制及临床用药提供科学依据,建立了清肺排毒汤高效液相色谱(HPLC)指纹图谱,并对其进行主成分分析研究。按照《贵州省新型冠状病毒肺炎中医药防治参考方案(第四版)》收载的方法制备12批清肺排毒汤供试品溶液,采用高效液相色谱仪进行测定。根据中药色谱指纹图谱相似度评价系统(2004 A版)建立清肺排毒汤的HPLC指纹图谱,并采用SPSS 18.0软件进行主成分分析。结果显示:12批清肺排毒汤指纹图谱中有30个共有峰,与对照品比对,确认24号峰为黄芩苷,12批清肺排毒汤指纹图谱与对照指纹图谱的相似度为0.980~0.997;方法学验证表明仪器精密度较好,并且方法重复性、稳定性较好;主成分分析共得到6个主成分,方差贡献率分别为56.566%,12.847%,9.364%,6.781%,4.800%,3.980%,累计方差贡献率达到94.337%。

In order to provide a scientific basis for the quality control and clinical application of medication of Qingfei Paidu decoction for anti-corona virus disease 2019(COVID-19),high performance liquid chromatography(HPLC)fingerprint of Qingfei Huedu decoction was established and principal component analysis was carried out.According to the method recorded on Reference Program for Traditional Chinese Medicine Prevention and Treatment of Corona Virus Disease 2019 in Guizhou Province(4th edition),12 batches of the test solutions of Qingfei Paidu decoction were prepared and determined by HPLC.The HPLC fingerprint of Qingfei Huedu decoction was established according to the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2004 A edition),and the principal component analysis was carried out by SPSS 18.0 software.The results showed that there were 30 common peaks in the fingerprints of 12 batches of Qingfei Paidu decoction.Compared with the reference substance,peak 24 was confirmed to be baicalin,and the similarity was in the range of 0.980-0.997 between fingerprints of 12 batches of Qingfei Paidu decoction and reference fingerprint.The precision of the instrument was good,and the repeatability and stability of this method were good by methodology validation.Six principal components were obtained by principal component analysis,and the variance contribution rates were 56.566%,12.847%,9.364%,6.781%,4.800%,3.980%,respectively,with the cumulative variance contribution rate reached 94.337%.