川芎嗪对阿尔茨海默病小鼠神经的保护作用

Tetramethylpyrazine protects neural in mouse model of Alzheimer’s disease

目的探讨川芎嗪(tetramethylpyrazine,TMP)对阿尔茨海默病(Alzheimer’s disease,AD)小鼠海马神经组织的保护作用。方法以C57BL/6小鼠作为对照组,APP/PS1双转基因小鼠作为模型组、TMP低、中、高剂量组灌胃20、40、60mg/kgTMP,1次/d,连续30d。Morris水迷宫实验检测小鼠学习记忆和空间探索能力,酶联免疫吸附法检测小鼠海马神经组织超氧化物歧化酶(superoxide dismutase,SOD)活性、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性、丙二醛(malonaldehyde,MDA)含量、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素(interleukin,IL)-1β、IL-10、β-淀粉样蛋白(β-amyloid protein,Aβ)_(1-40)、Aβ_(1-42)、β-淀粉样前体蛋白(β-amyloid precursor protein,β-APP)含量。结果与对照组比较,模型组内平均逃避潜伏期延长,平台停留时间、跨平台次数、SOD活性、GSH-Px活性降低(P<0.05),MDA含量、TNF-α、IL-1β、IL-10、Aβ_(1-40)、Aβ_(1-42)、β-APP含量上升(P<0.05)。与模型组比较,TMP低、中、高剂量组小鼠平均逃避潜伏期缩短,平台停留时间、跨平台次数、SOD活性、GSH-Px活性上升(P<0.05),MDA含量、TNF-α、IL-1β、IL-10、Aβ_(1-40)、Aβ_(1-42)、β-APP含量降低(P<0.05)。结论TMP能改善AD小鼠学习记忆能力,减轻海马神经组织氧化应激和炎症反应,减少Aβ含量。

Objective To explore the protective effect of tetramethylpyrazine(TMP)on hippocampal neural tissue in Alzheimer’s disease(AD)mice.Methods The C57BL/6 mice were used as the control group,the APP/PS1 double transgenic mice were used as the model group,and the TMP low,medium and high dose groups were given intragastric administration of 20,40,and 60mg/kg of TMP,once a day for 30 consecutive days.Morris water maze test was used to detect the learning and memory and space exploration ability of mice.Enzyme-linked immunosorbent assay was used to detect superoxide dismutase(SOD)activity,glutathione peroxidase(GSH-Px)activity,malonaldehyde(MDA)content,tumor necrosis factor-α(TNF-α),interleukin(IL-)-1β,IL-10,β-amyloid protein(Aβ)_(1-40),Aβ_(1-42),β-amyloid precursor protein(β-APP)content in mouse hippocampal nerve tissue.Results Compared with the control group,the escape latency of the mice in the model group was prolonged,and the platform residence time,the times of cross-platforms,SOD activity,and GSH-Px activity were reduced(P<0.05),MDA content,TNF-α,IL-1β,IL-10,Aβ_(1-40),Aβ_(1-42),β-APP content were increased(P<0.05).Compared with the model group,the escape latency of mice in the low,medium,and high dose groups of TMP was shortened,and the platform residence time,cross-platform times,SOD activity,and GSH-Px activity were increased(P<0.05),MDA content,TNF-α,IL-1β,IL-10,Aβ_(1-40),Aβ_(1-42),β-APP content increased(P<0.05).Conclusion TMP can improve the learning and memory ability of AD mice,reduce the oxidative stress and inflammation of hippocampal nerve tissue,and reduce the content of Aβ.