血清3-硝基酪氨酸在2型糖尿病患者体内的变化及与代谢指标的关系

Changes of Serum 3-Nitrotyrosine in Patients with Type 2 Diabetes Melli⁃tus and Its Relationship with Metabolic Indexes

目的 探讨血清3-硝基酪氨酸在2型糖尿病患者(type 2 diabetes mellitus, T2DM)体内的水平变化,同时分析3-硝基酪氨酸与代谢指标的关系。方法 选取2020年1月—2022年12月于保定市第一中心医院住院治疗的46例T2DM患者作为研究对象(T2DM组),另选取同期健康体检人群44例作为对照组(NC组)。收集两组一般临床资料,检测总胆固醇、三酰甘油、丙氨酸氨基转移酶、血肌酐、空腹血糖、糖化血红蛋白、空腹胰岛素、C肽等生化指标水平并计算胰岛素抵抗指数。使用酶联免疫吸附双抗体夹心测定法检测两组3-硝基酪氨酸血清的表达水平。采用Spearman相关性分析评估3-硝基酪氨酸与各指标之间的相关性。结果T2DM组体质指数、收缩压、三酰甘油、空腹血糖、糖化血红蛋白、胰岛素抵抗指数、C肽、空腹胰岛素、3-硝基酪氨酸水平较NC组升高,差异有统计学意义(P<0.05)。血清3-硝基酪氨酸水平较高组与水平较低组相比体质指数、收缩压、舒张压、空腹血糖、糖化血红蛋白、C肽、胰岛素抵抗指数差异有统计学意义(P<0.05)。Spearman相关性分析结果显示血清3-硝基酪氨酸水平与体质指数、收缩压、舒张压、空腹血糖、糖化血红蛋白、C肽、胰岛素抵抗指数水平呈正相关(P<0.05)。结论 T2DM患者血清3-硝基酪氨酸水平升高,可能参与了胰岛素抵抗,影响了T2DM的发生发展,这一结果可为今后T2DM患者的诊治提供新思路及新方向。

Objective To investigate the changes of serum 3-nitrotyrosine level in type 2 diabetes patients(T2DM),and to analyze the relationship between 3-nitrotyrosine and metabolic indicators.Methods 46 T2DM patients hospitalized in the First Central Hospital of Baoding City from January 2020 to December 2022 were selected as the study subjects(T2DM group),and 44 healthy people in the same period were selected as the control group(NC group).General clinical data of the two groups were collected,and biochemical indexes such as total cholesterol,triacylglycerol,alanine aminotransferase,serum creatinine,fasting blood glucose,glycated hemoglobin,fasting insulin and C-peptide were measured,and insulin resistance index was calculated.The expression level of 3-nitrotyrosine in serum of two groups was detected by enzyme-linked immunosorbent double antibody sandwich assay.Spearman correlation analysis was used to evaluate the correlation between 3-nitrotyrosine and each index.Results The levels of Body mass index,systolic blood pressure,triacylglycerol,fasting plasma glucose,Glycated hemoglobin,insulin resistance index,C-peptide,fasting insulin,and 3-nitrotyrosine in T2DM group were higher than those in NC group(P<0.05).There were statistically significant differences in Body mass index,systolic blood pressure,diastolic blood pressure,fasting plasma glucose,glycated hemoglobin,C-peptide and insulin resistance index between the group with higher serum 3-nitrotyrosine level and the group with lower serum 3-nitrotyrosine level(P<0.05).Spearman correlation analysis showed that serum 3-nitrotyrosine levels were positively correlated with Body mass index,systolic blood pressure,dia‐stolic blood pressure,fasting plasma glucose,glycated hemoglobin,C-peptide,and insulin resistance index(P<0.05).Conclusion The increase in serum 3-nitrotyrosine levels in T2DM patients may be involved in insulin resistance and affect the occurrence and development of T2DM.This result can provide new ideas and directions for the diagnosis and treatment of T2DM patients in the future.