铜死亡基因在自闭症谱系障碍免疫浸润中的生物信息学分析

Bioinformatics Analysis of Cuprotosis-Related Genes in Immune Infiltration in Autism Spectrum Disorder

目的自闭症谱系障碍(ASD)是一种异质性的神经发育性疾病,铜死亡是一种新型的细胞死亡方式,本研究将探索铜死亡在ASD中的作用、ASD潜在的生物标志物以及可能的治疗药物。方法从GEO数据库中检索符合条件的ASD相关数据集,对数据集消除批次效应后进行合并、筛选铜死亡相关基因、分析免疫浸润情况、构建列线图,使用Enrichr网站对ASD铜死亡基因进行GO、KEEG分析及药物预测。结果对GSE18123、GSE29691两个数据集消除批次效应、合并、筛选铜死亡基因。通过免疫浸润分析,本研究筛选出12个基因SLC31A1、PDHB、PDHA1、LIPT1、ATP7A、ATP7B、DBT、DLAT、DLD、DLST、FDX1、LIAS,作为新的标志物构建ASD诊断预测模型,并发现维A酸是ASD潜在有效的药物分子。结论铜死亡基因可能通过免疫细胞浸润干预ASD的进展。

Objective Autism spectrum disorder(ASD)is a heterogeneous neurodevelopmental disorder,and cuprotosis is a new form of cell death.This study will explore the role of cuprotosis in autism spectrum disorder,potential neoplasm markers of autism spectrum disorder and possible therapeutic drugs.Methods Eligible ASD related datasets were retrieved from the GEO database.After eliminating batch effects,the dataset was merged,cuprotosis-related genes were screened,immune infiltration was analyzed,and a column chart was constructed.The Enrichr website was used to perform GO,KEEG analysis,and drug prediction of ASD cuprotosis-related genes.Results Two datasets,GSE18123 and GSE29691,were used to eliminate batch effects,merge,and screen cuprotosis-related gene.Through immune infiltration analysis,SLC31A1,PDHB,PDHA1,LIPT1,ATP7A,ATP7B,DBT,DLAT,DLD,DLST,FDX1,LIAS were identified as new biomarkers to construct ASD diagnostic prediction models,and vitinoin as potentially effective drug molecule for autism spectrum disorder.Conclusion Cuprotosis-related genes may intervene in the progression of ASD through immune cell infiltration.