抗蛋白/细胞粘附的两性离子功能化聚对二甲苯薄膜

Zwitterions Functionalized Parylene for Protein/Cell Repulsion

目前生物电子器件材料在复杂的生物环境中容易粘附蛋白/细胞等,进而影响器件的长期稳定性。研究并制备了抗非特异性蛋白/细胞粘附的两性离子功能化聚对二甲苯薄膜材料。通过原子转移自由基聚合方法在具有溴引发剂的聚对二甲苯表面接枝两性离子基团得到该薄膜材料。探索了不同两性离子聚合物分子链——聚甲基丙烯酸甲酯磷酸胆碱(PMPC)、聚甲基丙烯酸甲酯磺酸甜菜碱(PMSB)、聚甲基丙烯酸甲酯羧酸甜菜碱(PMCB)修饰的聚对二甲苯抗蛋白和细胞粘附的能力,发现PMPC修饰的聚对二甲苯性能最为优异。此外,采用光刻技术制备了PMPC修饰的聚对二甲苯图案化的生物界面,实现了在空间上控制细胞粘附行为。为聚对二甲苯薄膜的表面抗炎症细胞粘附及控制神经细胞生长提供了可能性,在生物电子器件领域具有潜在的应用价值。

Biomaterials for bioelectronics are susceptible to the adhesion of proteins/cells,which will affect the chronic stability of devices.Here,Anti-fouling parylene films were studied and prepared to resist the adhesion of proteins or cells.Bromine functionalized parylene film was first deposited on parylene,and then,atom transfer radical polymerization was conducted to modify parylene with zwitterions.Three different types of zwitterionic polymers,poly(2-methacryloyloxyethyl phosphorylcholine)(PMPC),poly(2-methacryloyloxyethyl sulfobetaine)(PMSB)and poly(2-methacryloyloxyethyl carboxybetaine)(PMCB)were used to modify parylene,and their properties were studied.It was observed that PMPC-functionalized parylene showed the best antifouling properties.Moreover,the PMPC-functionalized parylene was also utilized to fabricate patterned bio-platform by a lithography-combined microfabrication for spatially guiding neural cell attachment.These zwitterionic functionalized parylene films,which can resist anti-inflammatory cells and control the growth of nerve cells,have a promising future in the field of bioelectronic devices.