基于Biolog FF技术解析辐射对产黑色素短梗霉代谢活性的影响

Effects of radiation on metabolic activities of Aureobasidium melanogenum based on biolog FF system

【目的】分析辐射对短梗霉代谢活性的影响,研究真菌耐辐射机理机制。【方法】分离鉴定一株产黑色素短梗霉,并进行菌株的^(60)Coγ射线照射试验。根据菌株的耐辐射能力,设置^(60)Coγ射线0、2500、5000和10000 Gy 4个辐射剂量,采用Biolog FF技术检测不同辐射下菌株对FF微平板中碳源的利用程度,分析辐射对产黑色素短梗霉代谢碳源活性的影响。【结果】不同辐射剂量下菌株AWCD值存在明显差异。随着辐射剂量的升高,细胞代谢活性明显下降,羧酸类和氨基酸的利用呈下降趋势,而碳水化合物的利用率呈上升趋势。随着辐射剂量增加,菌株对碳源的利用率发生了变化。在Biolog FF微平板95种碳源中,共有46个碳源被利用,其中7种碳源利用率随辐射剂量上升而增加。其中,D-核糖,蔗糖,D-阿拉伯糖,L-阿拉伯糖的利用率增加最为明显。【结论】辐射能明显降低产黑色素短梗霉的代谢活性,其在高剂量辐射下碳源利用类型会发生明显的改变,可能是该菌辐射应激和适应的一种有效机制。

【Objective】In order to analyze the effects of radiation on the metabolic activity of Aureobasidium melanogenum,this project aims to explore the mechanism of fungal radiation tolerance.【Methods】The experiment used^(60)Coγradiation,and set four radiation doses of 0,2,500,5,000,and 10,000 Gy.Biolog FF technology was used to detect the use of carbon source in FF microplate by A.melanogenum under different radiation doses,and analyze the impact on carbon metabolic activity of A.melanogenum by radiation.【Results】There were significant differences in AWCD values under different radiation doses.With the increase of radiation dose,the metabolic activity of cells decreased significantly.The utilization of carboxylic acids and amino acids showed a downward trend,while the utilization rates of carbohydrates an upward trend.At the same time,as the radiation dose increased,the utilization of carbon source changed.Among the 95 carbon sources in the Biolog FF microplates,a total of 46 carbon sources were used,and 7 carbon sources increased in average utilization rate with the increase of radiation dose.Among them,the utilization rate of D-ribose,sucrose,D-arabinose and L-arabinose was the most obvious.【Conclusion】Radiation can obviously reduce the metabolic activity of A.melanogenum,and the utilization of carbon source will change significantly under high doses of radiation,which may be an effective mechanism for its adaptation to radiation.