野山杏总黄酮对LPS诱导鸡肝炎的影响

Effects of total flavonoids of wild apricot on LPS-induced chicken hepatitis

【目的】研究野山杏总黄酮对LPS诱导鸡肝炎的影响。【方法】给予鸡腹腔注射5 mg/kg LPS制备急性肝损伤(ALI)模型,以生理盐水为空白对照组,吲哚美辛为阳性对照组和野山杏总黄酮低(50 mg/kg)、中(100 mg/kg)、高(200 mg/kg)剂量组为试验组,测定鸡体温、体重的变化和血液学参数及血液生化指标,观察鸡肝脏病理,分析野山杏总黄酮对鸡肝炎的影响。【结果】野山杏总黄酮各剂量组可不同程度降低LPS导致的体温升高;缓解了炎症造成的体重减轻;显著或极显著(P<0.05,P<0.01)逆转LPS导致的炎性细胞增加,其中野山杏总黄酮各剂量组对白细胞数的减少具有剂量依赖性,对淋巴细胞、粒细胞及中间细胞不呈现剂量依赖性;野山杏总黄酮各剂量组均可抑制炎症条件下ALT、AST的水平;中高剂量组能明显改善LPS引起的鸡肝组织病理损伤。【结论】野山杏总黄酮各剂量组均能恢复炎症导致的体温升高,对鸡体重有促进作用,能减少炎性细胞的数量,抑制ALT、AST含量升高,改善LPS致鸡肝炎的病理损伤,具有一定的抗肝炎作用。

【Objective】To study the effect of total flavone of wild apricot on lPS-induced chicken hepatitis.【Methods】Acute liver injury(ALI)model was established by intraperitoneal injection of LPS at 5 mg/kg in chickens and normal saline was used as blank control group,indomethacin as positive control group,and low(50 mg/kg),medium(100 mg/kg)and high(200 mg/kg)dose groups of total flavonoids of wild apricot were taken orally as experimental groups to analyze the effect of total flavonoids of wild apricot on chicken hepatitis.【Results】Total flavonoids of apricot in each dose group could reduce the temperature rise caused by LPS to different degrees and relieve weight loss caused by inflammation;The increase of inflammatory cells induced by LPS was significantly or extremely significantly reversed(P<0.05,P<0.01).The decrease of WBC was dose-dependent in each dose group,but not in lymphocyte,granulocyte and intermediate cells.The levels of ALT and AST in all dosage groups were inhibited.At the same time,the liver histopathological injury induced by LPS could be significantly improved in the medium and high dose group.【Conclusion】Total flavonoids of wild apricot in all dose groups can restore the increase of body temperature caused by inflammation and promote the weight of chickens,thus reducing the number of inflammatory cells,inhibiting the increase of ALT and AST and improving the pathological damage of chicken hepatitis caused by LPS,so conclusion can be reached that the total flavonoids of wild apricot can have a certain anti hepatitis effect,and be of a certain practical significance for the actual health of domestic poultry breeding.