HMGA1、sHLA-G联合TSGF检测在宫颈癌早期筛查及病情评估中的应用

Application of HMGA1,sHLA-G combined with TSGF detection in early screening and disease assessment of cervical cancer

目的分析血清高迁移率族蛋白A1(HMGA1)、可溶性白细胞G抗原(sHLA-G)联合恶性肿瘤特异生长因子(TSGF)检测在宫颈癌早期筛查及病情评估中的应用价值。方法选取2019年2月至2022年2月安徽医科大学第二附属医院收治的110例宫颈癌患者(恶性组),另选取本院同期收治的宫颈上皮内瘤变110例作为良性组,及健康体检女性110名作为对照组。受试对象均进行HMGA1、sHLA-G、TSGF检测。对比三组HMGA1、sHLA-G、TSGF表达水平,收集恶性组患者资料,对比不同临床分期、分化程度、淋巴结转移者HMGA1、sHLA-G、TSGF表达水平,绘制ROC曲线分析HMGA1、sHLA-G、TSGF联合检测对宫颈癌筛查的价值。结果HMGA1、sHLA-G、TSGF表达水平由高到低依次为恶性组>良性组>对照组,比较差异有统计学意义(P<0.05)。临床Ⅲ-Ⅳ期、低分化、淋巴结转移者HMGA1、sHLA-G、TSGF表达水平分别高于临床Ⅰ~Ⅱ期者、中-高分化、淋巴未转移者,差异有统计学意义(P<0.05)。依据ROC曲线可知,HMGA1、sHLA-G、TSGF对宫颈癌诊断AUC值分别为0.781(95%CI:0.699~0.861)、0.706(95%CI:0.607~0.804)、0.722(95%CI:0.629~0.816),HMGA1+sHLA-G+TSGF联合诊断的AUC值为0.813(95%CI:0.717~0.896),高于三者单独检测(P<0.05)。结论HMGA1、sHLA-G、TSGF表达水平与宫颈癌恶性程度相关,三者联合检测可提高宫颈癌早期筛查效能,或可作为宫颈癌筛查及诊断的指标应用于临床。

Objective To analyze the application value of serum high mobility group protein A1(HMGA1),soluble leucocyte G antigen(sHLA-G)and malignant tumor specific growth factor(TSGF)detection in early screening and disease assessment of cervical cancer.Methods 110 patients with cervical cancer(malignant group)were selected from our hospital,and another 110 women with cervical intraepithelial neoplasia and healthy physical examination were selected from our hospital from February 2019 to February 2022.They were divided into the benign group and the control group.All subjects were tested for HMGA1,sHLA-G,and TSGF.The expression levels of HMGA1,sHLA-G,and TSGF were compared among the three groups,and the data of patients in the malignant group were collected.The expression levels of HMGA1,sHLA-G,and TSGF were compared in patients with different clinical stages,differentiation degrees,and lymph node metastasis.The value of combined detection of TSGF in cervical cancer screening was analyzed.Results The expression levels of HMGA1,sHLA-G,and TSGF from high to low were malignant group>benign group>control group,and the difference was statistically significant(P<0.05).The expression levels of HMGA1,sHLA-G and TSGF in patients with clinical stage Ⅲ~Ⅳ,low differentiation and lymph node metastasis were higher than those in patients with clinical stage Ⅰ~Ⅱ,medium to high differentiation and lymph node metastasis,and the difference was statistically significant(P<0.05).According to the ROC curve,the AUC values of HMGA1,sHLA-G and TSGF for cervical cancer diagnosis were 0.781(95%CI:0.699~0.861),0.706(95%CI:0.607~0.804)and 0.722(95%CI:0.629~0.816),respectively,the AUC value of HMGA1+sHLA-G+TSGF combined diagnosis was 0.813(95%CI:0.717~0.896),which was higher than that of HMGA1+Shla-G+TSGF alone detection(P<0.05).Conclusion The expression levels of HMGA1,sHLA-G,and TSGF are related to the malignancy of cervical cancer.The combined detection of the three can improve the effectiveness of early screening of cervical cancer,or it can be used as an indicator for cervical cancer screening and diagnosis in clinical practice.