杜仲叶中绿原酸提取工艺优化及分离制备

Optimization of Extraction Process and Separation of Chlorogenic Acid from Eucommia ulmoides Leaves

目的:优化绿原酸提取工艺,并进行单体制备。方法:在单因素试验基础上,以料液比、乙醇浓度和pH为影响因素,绿原酸提取收率为评价指标,依据中心组合法则,运用3因素3水平的响应曲面分析法优化提取工艺。以绿原酸提取液为原料,采用高压制备液相色谱法分离绿原酸。结果:最优提取工艺为料液比1∶10(g/mL)、乙醇浓度20%、pH 3.5、提取时间90 min、提取温度75℃,绿原酸的平均提取率为2.78%,模型预测值为2.94%。高压制备液相分离获得的绿原酸单体纯度大于98.75%,收率大于95.0%。结论:建立了一种简便高效的绿原酸提取分离工艺。

Objective:To optimize the extraction process of chlorogenic acid and prepare monomers.Methods:On the basis of single factor experiment,the response surface methodology with three factors and three levels was used to optimize the extraction process according to the central combination rule,with the material liquid ratio,ethanol concentration and pH as the influencing factors,and the yield of chlorogenic acid as the evaluation index.Using chlorogenic acid extract as raw material,chlorogenic acid was separated by high pressure preparative liquid chromatography.Results:The optimal extraction process was as follows:material liquid ratio was 1∶10(g/mL),ethanol concentration was 20%,pH was 3.5,extraction time was 90 min,and extraction temperature was 75℃.The average extraction rate of chlorogenic acid was 2.78%,and the predicted value of the model was 2.94%.The purity of chlorogenic acid monomer obtained was more than 98.75%,and the yield was more than 95.0%.Conclusion:A simple and efficient extraction and separation process of chlorogenic acid was established.